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PMID: 1624431 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role of the Bacillus subtilis gsiA gene in regulation of early sporulation gene expression.

Journal of bacteriology ·Vol. 174 ·No. 13 ·1992-07-00 ·Pages 4374-83

Mueller JP, Sonenshein AL

Abstract

The Bacillus subtilis gsiA operon was induced rapidly, but transiently, as cells entered the stationary phase in nutrient broth medium. A mutation at the gsiC locus caused sporulation to be defective and expression of gsiA to be elevated and prolonged. The sporulation defect in this strain was apparently due to persistent expression of gsiA, since a gsiA null mutation restored sporulation to wild-type levels. Detailed mapping experiments revealed that the gsiC82 mutation lies within the kinA gene, which encodes the histidine protein kinase member of a two-component regulatory system. Since mutations in this gene caused a substantial blockage in expression of spoIIA, spoIIG, and spoIID genes, it seems that accumulation of a product of the gsiA operon interferes with sporulation by blocking the completion of stage II. It apparently does so by inhibiting or counteracting the activity of KinA.

MeSH Terms
Alleles Amino Acid Sequence Bacillus subtilis/genetics,physiology Bacterial Proteins/genetics Base Sequence Chromosomes, Bacterial Crossing Over, Genetic Gene Expression Regulation, Bacterial Genes, Bacterial Genotype Histidine Kinase Kinetics Models, Genetic Molecular Sequence Data Mutation Operon Protein Kinases/genetics Recombination, Genetic Restriction Mapping Spores, Bacterial/physiology Transcription, Genetic
Chemicals
Bacterial Proteins kinA protein, Bacillus subtilis Protein Kinases Histidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mueller J P
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Sonenshein A L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.13.4374-4383.1992
Published
1992-07-00
Pages
4374-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206222
Subset
IM
Grants
NIGMS NIH HHS · F32-GM13181 · United States
NIGMS NIH HHS · GM42219 · United States
Databases
GENBANK
M29450, M79367, M79368, M79369, M79370, M79371, M79372, M84980, X56680, Z11493
External Links
PubMed source DOI: 10.1128/jb.174.13.4374-4383.1992 PMC Full Text View journal details
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