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PMID: 11161045 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sugar coordinately and differentially regulates growth- and stress-related gene expression via a complex signal transduction network and multiple control mechanisms.

Plant physiology ·Vol. 125 ·No. 2 ·2001-02-00 ·Pages 877-90

Ho S, Chao Y, Tong W, Yu S

Abstract

In plants, sugars are required to sustain growth and regulate gene expression. A large set of genes are either up- or down-regulated by sugars; however, whether there is a common mechanism and signal transduction pathway for differential and coordinated sugar regulation remain unclear. In the present study, the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system to address this question. Sucrose and glucose both played dual functions in gene regulation as exemplified by the up-regulation of growth-related genes and down-regulation of stress-related genes. Sugar coordinately but differentially activated or repressed gene expression, and nuclear run-on transcription and mRNA half-life analyses revealed regulation of both the transcription rate and mRNA stability. Although coordinately regulated by sugars, these growth- and stress-related genes were up-regulated or down-regulated through hexokinase-dependent and/or hexokinase-independent pathways. We also found that the sugar signal transduction pathway may overlap the glycolytic pathway for gene repression. alpha-Amylase and the stress-related genes identified in this study were coordinately expressed under sugar starvation, suggesting a convergence of the nutritional and environmental stress signal transduction pathways. Together, our studies provide a new insight into the complex signal transduction network and mechanisms of sugar regulation of growth and stress-related genes in plants.

MeSH Terms
DNA, Complementary/genetics Gene Expression Regulation, Plant/drug effects,physiology Gene Library Oryza/genetics,growth & development Plasmids RNA Processing, Post-Transcriptional/drug effects RNA, Messenger/genetics Signal Transduction/physiology Sucrose/pharmacology Transcription, Genetic/drug effects
Chemicals
DNA, Complementary RNA, Messenger Sucrose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ho S
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China.
Chao Y
Tong W
Yu S
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.125.2.877
Published
2001-02-00
Pages
877-90
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC64889
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.125.2.877 PMC Full Text View journal details
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