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PMID: 2101689 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural characterization of a rice actin gene.

Plant molecular biology ·Vol. 14 ·No. 2 ·1990-02-00 ·Pages 163-71

McElroy D, Rothenberg M, Wu R

Abstract

We have isolated and sequenced a full-length cDNA clone containing information for the rice actin gene RAc1. Transcript terminus mapping and sequence alignment between the RAc1 cDNA clone and a previously isolated RAc1 genomic clone were used to determine the structure of the RAc1 gene. This allowed us to make the first complete structural characterization of a plant actin gene. The analysis revealed the presence of a 5'-noncoding exon, separated by an intron, from the first translated exon of the RAc1 gene. This is one of the few reported cases of a plant gene containing such a 5'-noncoding exon. Sequence comparison between the previously isolated plant actin genes suggests that such an exon may be a common feature of plant actin gene structure. The present study also confirms that the rice actin gene family is composed of at least eight unique members.

Related Genes
Dma2 Mac1 Pac&psgr Rac1 Sac3 XAc&bgr
MeSH Terms
Actins/genetics Amino Acid Sequence Animals Cloning, Molecular Genes, Plant Humans Introns Molecular Sequence Data Oligonucleotide Probes Oryza/genetics Pseudogenes Rats Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Actins Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McElroy D
Field of Botany, Cornell University, Ithaca, NY 14853.
Rothenberg M
Wu R
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23 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00018557
Published
1990-02-00
Pages
163-71
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Grants
NIGMS NIH HHS · GM29179 · United States
External Links
PubMed source DOI: 10.1007/BF00018557 View journal details
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