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PMID: 3428258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A complex gene superfamily encodes actin in petunia.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3223-31

Baird WV, Meagher RB

Abstract

We have shown by several independent criteria that actin is encoded by a very large and complex superfamily of genes in Petunia. Several cDNA and genomic probes encoding actins from diverse organisms (Dictyostelium, Drosophila, chicken and soybean) hybridize to hundreds of restriction fragments in the petunia genome. Actin-hybridizing sequences were isolated from a petunia genomic library at a rate of at least 200 per genome equivalent. Twenty randomly selected actin-hybridizing clones were characterized in more detail. DNA sequence data from four representative and highly divergent clones, PAc2, PAc3, PAc4 and PAc7, demonstrate that these actin-like sequences are related to functional actin genes. Intron positions typical of other known plant actin genes are conserved in these clones. Four of six clones analyzed (PAc1, PAc2, PAc3, PAc4) hybridize to leaf mRNA of the same size (1.7 kb) as that reported for other plant actin mRNAs and to a slightly smaller mRNA species (1.5 kb). Five distinct subfamilies of actin-related genes were characterized which varied in size from a few members to several dozen members. It is clear from our data that other actin gene subfamilies must also exist within the genome. Possible mechanisms of actin gene amplification and genome turnover are discussed.

MeSH Terms
Actins/genetics Amino Acid Sequence DNA/analysis Exons Gene Amplification Genes Molecular Sequence Data Nucleic Acid Hybridization Plants/genetics RNA, Messenger/genetics Transcription, Genetic
Chemicals
Actins RNA, Messenger DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baird W V
Department of Genetics, University of Georgia, Athens 30602.
Meagher R B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3223-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553773
Subset
IM
Grants
NIGMS NIH HHS · GM36397 · United States
External Links
PubMed source PMC Full Text View journal details
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