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PMID: 4000121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Novel chicken actin gene: third cytoplasmic isoform.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1151-62

Bergsma DJ, Chang KS, Schwartz RJ

Abstract

We identified a novel chicken actin gene. The actin protein deduced from its nucleotide sequence very closely resembles the vertebrate cytoplasmic actins; accordingly, we classified this gene as a nonmuscle type. We adopted the convention for indicating the nonmuscle actins of the class Amphibia (Vandekerckhove et al., J. Mol. Biol. 152:413-426) and denoted this gene as type 5. RNA blot analysis demonstrated that the type 5 actin mRNA transcripts accumulate in adult tissues in a pattern indicative of a nonmuscle actin gene. Genomic DNA blots indicated that the type 5 actin is a single copy gene and a distinct member of the chicken actin multigene family. Inspection of the nucleotide sequence revealed many features that distinguished the type 5 gene from all other vertebrate actin genes examined to date. These unique characteristics include: (i) an initiation Met codon preceding an Ala codon, a feature previously known only in plant actins, (ii) a single intron within the 5' untranslated region, with no interruptions in the coding portion of the gene, and (iii) an atypical Goldberg-Hogness box (ATAGAA) preceding the mRNA initiation terminus. These unusual features have interesting implications for actin gene diversification during evolution.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Brain/physiology Chickens/genetics Cytoplasm/metabolism Gene Expression Regulation Genes Genetic Linkage Nucleic Acid Hybridization Promoter Regions, Genetic Protein Biosynthesis RNA, Messenger/genetics Tissue Distribution Transcription, Genetic
Chemicals
Actins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bergsma D J
Chang K S
Schwartz R J
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62 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1151-1162.1985
Published
1985-05-00
Pages
1151-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366834
Subset
IM
Grants
NINDS NIH HHS · NS-15050 · United States
Databases
GENBANK
K02255, M10279
External Links
PubMed source DOI: 10.1128/mcb.5.5.1151-1162.1985 PMC Full Text View journal details
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