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PMID: 6513927 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of six different chicken actin genes.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2498-508

Chang KS, Zimmer WE, Bergsma DJ, Dodgson JB, Schwartz RJ

Abstract

Genes representing six different actin isoforms were isolated from a chicken genomic library. Cloned actin cDNAs as well as tissue-specific mRNAs enriched in different actin species were used as hybridization probes to group individual actin genomic clones by their relative thermal stability. Restriction maps showed that these actin genes were derived from separate and nonoverlapping regions of genomic DNA. Of the six isolated genes, five included sequences from both the 5' and 3' ends of the actin-coding area. Amino acid sequence analysis from both the NH2- and COOH-terminal regions provided for the unequivocal identification of these genes. The striated isoforms were represented by the isolated alpha-skeletal, alpha-cardiac, and alpha-smooth muscle actin genes. The nonmuscle isoforms included the beta-cytoplasmic actin gene and an actin gene fragment which lacked the 5' coding and flanking sequence; presumably, this region of DNA was removed from this gene during construction of the genomic library. Unexpectedly, a third nonmuscle chicken actin gene was found which resembled the amphibian type 5 actin isoform (J. Vandekerckhove, W. W. Franke, and K. Weber, J. Mol. Biol., 152:413-426). This nonmuscle actin type has not been previously detected in warm-blooded vertebrates. We showed that interspersed, repeated DNA sequences closely flanked the alpha-skeletal, alpha-cardiac, beta-, and type 5-like actin genes. The repeated DNA sequences which surround the alpha-skeletal actin-coding regions were not related to repetitious DNA located on the other actin genes. Analysis of genomic DNA blots showed that the chicken actin multigene family was represented by 8 to 10 separate coding loci. The six isolated actin genes corresponded to 7 of 11 genomic EcoRI fragments. Only the alpha-smooth muscle actin gene was shown to be split by an EcoRI site. Thus, in the chicken genome each actin isoform appeared to be encoded by a single gene.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Chickens/genetics Chromosome Mapping Cloning, Molecular DNA/genetics Genes Repetitive Sequences, Nucleic Acid
Chemicals
Actins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chang K S
Zimmer W E
Bergsma D J
Dodgson J B
Schwartz R J
References (49)
49 references, click to expand
  1. Human cytoplasmic actin proteins are encoded by a multigene family.
    Mol Cell Biol. 1982 Jun;2(6):674-84 PMID: 14582162
  2. Presence of a highly repetitive and widely dispersed DNA sequence in the human genome.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1508-12 PMID: 6262808
  3. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  4. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Identification and characterization of multiple forms of actin.
    Cell. 1976 Dec;9(4 PT 2):793-805 PMID: 1017015
  7. Hemoglobin switching in sheep and goats. Preparation and characterization of complementary DNAs specific for the alpha-, beta-, and gamma-globin messenger RNAs of sheep.
    J Biol Chem. 1977 Mar 25;252(6):1908-16 PMID: 845153
  8. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  9. Purification and characterization of covalently closed replicative intermediates of ColEl DNA from Escherichia coli.
    Biochemistry. 1977 Apr 19;16(8):1677-83 PMID: 322703
  10. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  11. Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1106-10 PMID: 274701
  12. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  13. Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genes.
    Cell. 1978 Nov;15(3):763-78 PMID: 728989
  14. Regulation of gene expression: possible role of repetitive sequences.
    Science. 1979 Jun 8;204(4397):1052-9 PMID: 451548
  15. Vitellogenin in Xenopus laevis is encoded in a small family of genes.
    Cell. 1979 Mar;16(3):535-49 PMID: 455441
  16. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library.
    Cell. 1979 Aug;17(4):879-87 PMID: 158428
  17. The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.
    Differentiation. 1979;14(3):123-33 PMID: 499690
  18. Differential expression of gizzard actin genes during chick embryogenesis.
    J Biol Chem. 1979 Nov 10;254(21):11119-25 PMID: 500629
  19. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  20. Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families.
    Cell. 1979 Dec;18(4):1303-16 PMID: 519770
  21. The actin genes of Drosophila: a dispersed multigene family.
    Cell. 1980 Feb;19(2):365-78 PMID: 6244106
  22. The organization of repetitive sequences in a cluster of rabbit beta-like globin genes.
    Cell. 1980 Feb;19(2):379-91 PMID: 6244107
  23. Ubiquitous, interspersed repeated sequences in mammalian genomes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398-402 PMID: 6246492
  24. Regulation of muscle differentiation: isolation and purification of chick actin messenger ribonucleic acid and quantitation with complementary deoxyribonucleic acid probes.
    Biochemistry. 1980 May 27;19(11):2506-14 PMID: 6892992
  25. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
    Cell. 1980 May;20(1):95-105 PMID: 6893015
  26. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2546-50 PMID: 6994099
  27. Pseudogenes.
    Nature. 1980 Aug 28;286(5776):840-1 PMID: 7412866
  28. A simple, rapid, and sensitive DNA assay procedure.
    Anal Biochem. 1980 Mar 1;102(2):344-52 PMID: 6158890
  29. Analysis of the regions flanking the human insulin gene and sequence of an Alu family member.
    Nucleic Acids Res. 1980 Sep 25;8(18):4091-109 PMID: 6253909
  30. The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA.
    Nucleic Acids Res. 1980 Mar 25;8(6):1201-15 PMID: 7433120
  31. Nucleotide sequences of 4.5S RNAs associated with poly(A)-containing RNAs of mouse and hamster cells.
    Nucleic Acids Res. 1980 Mar 25;8(6):1273-85 PMID: 6159592
  32. Regulation of muscle differentiation: cloning of sequences from alpha-actin messenger ribonucleic acid.
    Biochemistry. 1980 Dec 9;19(25):5883-90 PMID: 7006694
  33. Multiple alpha and beta tubulin genes represent unlinked and dispersed gene families.
    J Biol Chem. 1981 Mar 25;256(6):3130-4 PMID: 6259149
  34. Actin typing on total cellular extracts: a highly sensitive protein-chemical procedure able to distinguish different actins.
    Eur J Biochem. 1981 Jan;113(3):595-603 PMID: 6783401
  35. The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not.
    Cell. 1981 Apr;24(1):107-16 PMID: 6263481
  36. Vascular smooth muscle cells differ from other smooth muscle cells: predominance of vimentin filaments and a specific alpha-type actin.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):298-302 PMID: 7017714
  37. Gene switching in myogenesis: differential expression of the chicken actin multigene family.
    Biochemistry. 1981 Jul 7;20(14):4122-9 PMID: 7284314
  38. There are approximately 20 actin gene in the human genome.
    Nucleic Acids Res. 1981 Oct 10;9(19):4895-908 PMID: 6273789
  39. Definition of 5' and 3' structural boundaries of the chromatin domain containing the ovalbumin multigene family.
    J Biol Chem. 1982 Feb 10;257(3):1501-7 PMID: 6276388
  40. Diversity of expression of non-muscle actin in amphibia.
    J Mol Biol. 1981 Oct 25;152(2):413-26 PMID: 6977037
  41. The complete nucleotide sequence of the chick a-actin gene and its evolutionary relationship to the actin gene family.
    Nucleic Acids Res. 1982 Jul 10;10(13):3861-76 PMID: 6287424
  42. Number and organization of actin-related sequences in the mouse genome.
    J Mol Biol. 1983 Jun 15;167(1):77-101 PMID: 6306256
  43. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
    Mol Cell Biol. 1983 May;3(5):787-95 PMID: 6865942
  44. Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution.
    Mol Cell Biol. 1983 Oct;3(10):1783-91 PMID: 6646124
  45. Gene product of v-fgr onc: hybrid protein containing a portion of actin and a tyrosine-specific protein kinase.
    Science. 1984 Jan 6;223(4631):63-6 PMID: 6318314
  46. The nucleotide sequence of the chick cytoplasmic beta-actin gene.
    Nucleic Acids Res. 1983 Dec 10;11(23):8287-301 PMID: 6324080
  47. Organization and expression of multiple actin genes in the sea urchin.
    Mol Cell Biol. 1981 Jul;1(7):609-28 PMID: 9279375
  48. Transcriptional analysis of interspersed repetitive polymerase III transcription units in human DNA.
    Nucleic Acids Res. 1981 Mar 11;9(5):1171-89 PMID: 7232214
  49. Gene regulation for higher cells: a theory.
    Science. 1969 Jul 25;165(3891):349-57 PMID: 5789433
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2498-2508.1984
Published
1984-11-00
Pages
2498-508
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369081
Subset
IM
Grants
NINDS NIH HHS · NS-15050 · United States
NINDS NIH HHS · NS-16494 · United States
Databases
GENBANK
J00805, K02172, K02255, K02256, K02257, K02258, K02259, M10279, X02212
External Links
PubMed source DOI: 10.1128/mcb.4.11.2498-2508.1984 PMC Full Text View journal details
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