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PMID: 6262808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Presence of a highly repetitive and widely dispersed DNA sequence in the human genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1508-12

Tashima M, Calabretta B, Torelli G, Scofield M, Maizel A, Saunders GF

Abstract

A genomic DNA library consisting of human DNA fragments about 18 kilobases long cloned in a bacteriophage lambda vector was found to contain a specific repeated DNA segment. The repeated sequence is present in greater than 95% of the genomic library, and selected clones contain at least two copies of the sequence. Our experiments indicate that this highly repetitive sequence (approximately 400,000 copies per haploid genome) is widely distributed in the human genome and is represented in the cytoplasmic polysomal mRNA. This sequence is homologous to the 300-base-pair Alu repeat family, the predominant repeat sequence in man.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Humans Molecular Weight Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tashima M
Calabretta B
Torelli G
Scofield M
Maizel A
Saunders G F
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1508
Published
1981-03-00
Pages
1508-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319160
Subset
IM
Grants
NCI NIH HHS · CA 16672 · United States
NCI NIH HHS · CA 20214 · United States
NIGMS NIH HHS · GM 23965 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1508 PMC Full Text View journal details
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