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PMID: 6893748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and developmental expression of the chick alpha-actin gene.

Nucleic acids research ·Vol. 8 ·No. 21 ·1980-11-11 ·Pages 4989-5005

Ordahl CP, Tilghman SM, Ovitt C, Fornwald J, Largen MT

Abstract

Recombinant DNA clones containing chick alpha-actin mRNA sequence have been isolated and used as probes to analyze the structure and developmental expression of the chick alpha-actin gene. The full length, 2000 nucleotide alpha-actin mRNA is detected in poly(A) RNA at early and late stages of in vivo leg muscle development. As expected, the alpha-actin mRNA is present at very low levels at early myogenic stages but is a high abundance species in terminally differentiated muscle. However, most of the alpha-actin mRNA from fused leg muscle is shorter than 2000 nucleotides, and occurs in relatively discrete size classes. An alpha-actin-like mRNA can be detected in poly(A) RNA from early embryonic brain, indicating that transcription of the alpha-actin gene may not be strictly muscle-specific at all stages of development. We have identified at least 3, very short (< 100 base pairs) intervening sequences in the alpha-actin gene which was isolated from a chick genomic library. The structure of the chick alpha-actin gene differs, therefore, from the structures of actin genes from yeast and Drosophila, both of which contain a single, relatively long, intervening sequence.

MeSH Terms
Actins/genetics Animals Cell Differentiation Chickens/genetics Cloning, Molecular Gene Expression Regulation Molecular Weight Muscles/embryology RNA, Messenger/genetics
Chemicals
Actins RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ordahl C P
Tilghman S M
Ovitt C
Fornwald J
Largen M T
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.21.4989
Published
1980-11-11
Pages
4989-5005
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324274
Subset
IM
Grants
NIGMS NIH HHS · GM25400 · United States
External Links
PubMed source DOI: 10.1093/nar/8.21.4989 PMC Full Text View journal details
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