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PMID: 7784506 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analog and tissue-specific expression suggest a role in sink-source regulation.

Plant physiology ·Vol. 108 ·No. 1 ·1995-05-00 ·Pages 285-94

Roitsch T, Bittner M, Godt DE

Abstract

Photoautotrophic suspension-culture cells of Chenopodium rubrum that were shifted to mixotrophic growth by adding glucose were used as model system to investigate the influence of the source-sink transition in higher plants on the expression and enzyme activities of intracellular and extracellular invertases. The complete cDNA coding for an extracellular invertase was cloned and sequenced from C. rubrum, and its identity has been proven by heterologous expression in Saccharomyces cerevisiae. The higher activity of extracellular invertase after preincubation in the presence of glucose was paralleled by an increased expression of the corresponding gene. The induction by glucose could be mimicked by the nonmetabolizable glucose analog 6-deoxyglucose. Both enzyme activity and mRNA level of extracellular invertase showed a sink-tissue-specific distribution in plants. The activity of neutral and acidic intracellular invertases were not affected by preincubation of autotrophic tissue cultures with sugars, nor did they show a tissue-specific distribution in plants. The data suggest that apoplastic invertase not only has an important function in phloem unloading and carbohydrate partitioning between source and sink tissues but may also have a role in establishing metabolic sinks.

Related Genes

CIN1 CIN2

MeSH Terms

Amino Acid Sequence Base Sequence Cells, Cultured Cloning, Molecular DNA Primers DNA, Complementary Deoxyglucose/analogs & derivatives,pharmacology Enzyme Induction/drug effects Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glucose/pharmacology Glycoside Hydrolases/biosynthesis,metabolism Kinetics Molecular Sequence Data Organ Specificity Phylogeny Plants/drug effects,enzymology,genetics Polymerase Chain Reaction Saccharomyces cerevisiae Sequence Homology, Amino Acid beta-Fructofuranosidase

Chemicals

DNA Primers DNA, Complementary Deoxyglucose Glycoside Hydrolases beta-Fructofuranosidase Glucose 6-deoxyglucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Roitsch T

Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.

Bittner M

Godt D E

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.108.1.285

Published

1995-05-00

Pages

285-94

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC157333

Subset

Databases

GENBANK

X81792, X81793, X81794, X81795, X81796, X81797

External Links

PubMed source DOI: 10.1104/pp.108.1.285 PMC Full Text View journal details

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Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analog and tissue-specific expression suggest a role in sink-source regulation.

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