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PMID: 9014361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Hexokinase as a sugar sensor in higher plants.

The Plant cell ·Vol. 9 ·No. 1 ·1997-01-00 ·Pages 5-19

Jang JC, León P, Zhou L, Sheen J

Abstract

The mechanisms by which higher plants recognize and respond to sugars are largely unknown. Here, we present evidence that the first enzyme in the hexose assimilation pathway, hexokinase (HXK), acts as a sensor for plant sugar responses. Transgenic Arabidopsis plants expressing antisense hexokinase (AtHXK) genes are sugar hyposensitive, whereas plants overexpressing AtHXK are sugar hypersensitive. The transgenic plants exhibited a wide spectrum of altered sugar responses in seedling development and in gene activation and repression. Furthermore, overexpressing the yeast sugar sensor YHXK2 caused a dominant negative effect by elevating HXK catalytic activity but reducing sugar sensitivity in transgenic plants. The result suggests that HXK is a dual-function enzyme with a distinct regulatory function not interchangeable between plants and yeast.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/enzymology,genetics,growth & development Carbohydrate Metabolism Carbohydrates/pharmacology Chromosome Mapping Gene Expression Genes, Plant Genetic Complementation Test Hexokinase/genetics,metabolism Humans Molecular Sequence Data Plants, Genetically Modified Rats Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Amino Acid Species Specificity
Chemicals
Carbohydrates Hexokinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jang J C
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA.
León P
Zhou L
Sheen J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.9.1.5
Published
1997-01-00
Pages
5-19
Language
English
Region
England
NLM ID
9208688
PMCID
PMC156897
Subset
IM
Databases
GENBANK
U28214, U28215
External Links
PubMed source DOI: 10.1105/tpc.9.1.5 PMC Full Text View journal details
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