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PMID: 8321195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis.

Molecular and cellular biology ·Vol. 13 ·No. 7 ·1993-07-00 ·Pages 3882-9

Prior C, Mamessier P, Fukuhara H, Chen XJ, Wesolowski-Louvel M

Abstract

The RAG1 gene of Kluyveromyces lactis encodes a low-affinity glucose/fructose transporter. Its transcription is induced by glucose, fructose, and several other sugars. The RAG4, RAG5, and RAG8 genes are trans-acting genes controlling the expression of the RAG1 gene. We report here the characterization of one of these genes, RAG5. The nucleotide sequence of the cloned RAG5 gene indicated that it encodes a protein that is homologous to hexokinases of Saccharomyces cerevisiae. rag5 mutants showed no detectable hexokinase or glucokinase activity, suggesting that the sugar kinase activity encoded by this gene is the only hexokinase in K. lactis. Both high- and low-affinity transport systems of glucose were affected in rag5 mutants. The defect of the low-affinity component was found to be due to a block of transcription of the RAG1 gene by the hexokinase mutation. In vivo complementation of the rag5 mutation by the HXK2 gene of S. cerevisiae and complementation of hxk1 hxk2 mutations of S. cerevisiae by the RAG5 gene showed that RAG5 and HXK2 were equivalent for sugar-phosphorylating activity but that RAG5 could not restore glucose repression in the S. cerevisiae hexokinase mutants.

Related Genes
HXK2 RAG1 RAG5
MeSH Terms
Amino Acid Sequence Blotting, Northern Cloning, Molecular Gene Expression Regulation, Fungal/genetics Genes, Fungal Genetic Complementation Test Hexokinase/genetics,metabolism Kluyveromyces/enzymology,genetics,growth & development Molecular Sequence Data Monosaccharide Transport Proteins/genetics,metabolism Phenotype Phosphorylation Restriction Mapping Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Monosaccharide Transport Proteins Hexokinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Prior C
Section de Biologie, Institut Curie, Centre Universitaire, Orsay, France.
Mamessier P
Fukuhara H
Chen X J
Wesolowski-Louvel M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.7.3882-3889.1993
Published
1993-07-00
Pages
3882-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359921
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.7.3882-3889.1993 PMC Full Text View journal details
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