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PMID: 16668448 Published · ppublish English Journal Article

Two Apoplastic alpha-Amylases Are Induced in Tobacco by Virus Infection.

Plant physiology ·Vol. 97 ·No. 2 ·1991-10-00 ·Pages 651-6

Heitz T, Geoffroy P, Fritig B, Legrand M

Abstract

alpha-Amylase activity (EC 3.2. 1.1) is greatly increased in leaves of tobacco (Nicotiana tabacum L. cv Samsun NN) infected with tobacco mosaic virus (TMV). The kinetics of enzyme induction during the hypersensitive reaction resemble those of other hydrolases known to be pathogenesis-related proteins of tobacco. Two alpha-amylases were purified from TMV-infected leaves and shown to have features in common with well-characterized pathogenesis-related proteins: they are acidic monomers that can be separated upon electrophoresis on basic native gels, and they are found in the apoplastic compartment of the cell. This extra-cellular localization was demonstrated by comparing the alpha-amylase partition between the intercellular wash fluid and the cell extract with that of proteins of known cellular compartmentalization. These data indicate an active secretion of both alpha-amylases produced in tobacco upon TMV infection.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Heitz T
Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université Louis Pasteur, 12 rue du Général Zimmer 67084 Strasbourg Cédex, France.
Geoffroy P
Fritig B
Legrand M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.97.2.651
Published
1991-10-00
Pages
651-6
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1081056
External Links
PubMed source DOI: 10.1104/pp.97.2.651 PMC Full Text View journal details
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