Home LiteratureArticle Details
PMID: 16453802 Published · ppublish English Journal Article

Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3209-12

Kauffmann S, Legrand M, Geoffroy P, Fritig B

Abstract

Three of the ten acidic ;pathogenesis-related' (PR) proteins known to accumulate in Nicotiana tabacum cv Samsun NN reacting hypersensitively to tobacco mosaic virus, namely -O, -N and -2, have been shown to have 1,3-beta-glucanase (EC 3.2.1.39) activity. By using sera raised against each protein purified to homogeneity close serological relationships have been demonstrated between the three proteins. The same specific sera cross-reacted with a basic protein which is also a 1,3-beta-glucanase induced by virus infection and which can be considered as a new basic pathogenesis-related protein of tobacco. Protein PR-O and the basic 1,3-beta-glucanase display about the same specific enzymatic activity, i.e. 50-fold and 250-fold higher than specific activities of proteins PR-N and -2 respectively.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kauffmann S
Institut de Biologie Moléculaires des Plantes, 12, rue du Général Zimmer, 67000 Strasbourg, France.
Legrand M
Geoffroy P
Fritig B
References (19)
19 references, click to expand
  1. 1,3-beta-D-glucanases from Pisum sativum seedlings. I. Isolation and purification.
    Biochim Biophys Acta. 1979 Dec 7;571(2):244-55 PMID: 41581
  2. A -I,3-glucan hydrolase from Nicotiana glutinosa. II. Specificity, action pattern and inhibitor studies.
    Biochim Biophys Acta. 1972 Jan 20;258(1):248-64 PMID: 5058402
  3. 1,3-beta-D-glucanases from Pisum sativum seedlings. II. Substrate specificities and enzymic action patterns.
    Biochim Biophys Acta. 1979 Dec 7;571(2):256-69 PMID: 508768
  4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  5. Synthesis and localization of pathogenesis-related proteins in tobacco.
    Mol Cell Biol. 1987 Apr;7(4):1580-3 PMID: 3299048
  6. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN". II. Changes in protein constitution after infection with tobacco mosaic virus.
    Virology. 1970 Feb;40(2):190-211 PMID: 4191688
  7. Effect of infection with TMV and other viruses on the level of a -1,3-glucan hydrolase in leaves of Nicotiana glutinosa.
    Virology. 1972 Dec;50(3):791-8 PMID: 4640688
  8. Cell wall chemistry, morphogenesis, and taxonomy of fungi.
    Annu Rev Microbiol. 1968;22:87-108 PMID: 4879523
  9. [Hypersensitivity to viruses, temperature and soluble proteins in Nicotiana Xanthi n.c. Appearance of new macromolecules at the repression of viral synthesis].
    C R Acad Sci Hebd Seances Acad Sci D. 1970 May 11;270(19):2383-6 PMID: 4997619
  10. beta-1,3-Endoglucanase from Soybean Releases Elicitor-Active Carbohydrates from Fungus Cell Walls.
    Plant Physiol. 1983 Mar;71(3):460-5 PMID: 16662849
  11. A -I,3-glucan hydrolase from Nicotiana glutinosa. I. Extraction, purification and physical properties.
    Biochim Biophys Acta. 1972 Jan 20;258(1):238-47 PMID: 5061994
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the "pathogenesis-related" protein p 14.
    Arch Virol. 1982;74(2-3):181-96 PMID: 6891893
  14. Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco.
    EMBO J. 1985 Sep;4(9):2167-71 PMID: 15938049
  15. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):89-93 PMID: 16593796
  16. Preparation and purification of glucanase and chitinase from bean leaves.
    Plant Physiol. 1971 Jan;47(1):129-34 PMID: 16657567
  17. Host-Pathogen Interactions: XVI. PURIFICATION AND CHARACTERIZATION OF A beta-GLUCOSYL HYDROLASE/TRANSFERASE PRESENT IN THE WALLS OF SOYBEAN CELLS.
    Plant Physiol. 1981 Jul;68(1):207-20 PMID: 16661872
  18. Host-Pathogen Interactions : XVII. HYDROLYSIS OF BIOLOGICALLY ACTIVE FUNGAL GLUCANS BY ENZYMES ISOLATED FROM SOYBEAN CELLS.
    Plant Physiol. 1981 Jul;68(1):221-8 PMID: 16661873
  19. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3209-12
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553771
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2