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PMID: 10982332 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Promoter-specific targeting of human SWI-SNF complex by Epstein-Barr virus nuclear protein 2.

Journal of virology ·Vol. 74 ·No. 19 ·2000-10-00 ·Pages 8893-903

Wu DY, Krumm A, Schubach WH

Abstract

The multiprotein human SWI-SNF (hSWI-SNF) complex is a chromatin-remodeling machine that facilitates transcription by overcoming chromatin-mediated gene repression. We had previously shown that hSNF5/INI1, an intrinsic, consistent component of the hSWI/SNF complex, is associated with Epstein-Barr nuclear antigen 2 (EBNA2) and have proposed that EBNA2 directs this complex to key EBNA2-responsive viral and cellular genes. Using chromatin immunoprecipitation and quantitative PCR, we show that antibodies directed against components of the hSWI-SNF complex preferentially precipitate chromatin-associated DNA that contains a targeted EBNA2-responsive element in the context of both episomal and cellular chromatin. This enrichment does not occur in EBNA2-negative cells or when the EBNA2-responsive element is mutated. The stable association of the hSWI-SNF complex with the EBNA2-responsive promoter can also be disrupted by deletion of the TATA element, suggesting that EBNA2 in itself is insufficient to mediate stable targeting of the hSWI-SNF complex. These results demonstrate that recruitment of the hSWI-SNF complex to selected promoters can occur in vivo through its interaction with site-specific activator proteins and that stable targeting may require the presence of basal transcription factors.

MeSH Terms
Base Sequence Cell Transformation, Viral Chromatin/genetics Epstein-Barr Virus Nuclear Antigens Gene Expression Regulation, Viral Herpesvirus 4, Human Humans Molecular Sequence Data Nuclear Proteins/genetics Viral Proteins/genetics
Chemicals
Chromatin EBNA-2 protein, Human herpesvirus 4 Epstein-Barr Virus Nuclear Antigens Nuclear Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu D Y
Division of Medical Oncology, Department of Medicine, Veterans Administration Puget Sound Health Care System, Seattle Division, Seattle, Washington 98108, USA.
Krumm A
Schubach W H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.19.8893-8903.2000
Published
2000-10-00
Pages
8893-903
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC102084
Subset
IM
Grants
NCI NIH HHS · CA54337 · United States
NCI NIH HHS · CA719829 · United States
NCI NIH HHS · CA82459 · United States
External Links
PubMed source DOI: 10.1128/jvi.74.19.8893-8903.2000 PMC Full Text View journal details
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