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PMID: 10535918 Published · ppublish English Journal Article

Targeting a SWI/SNF-related chromatin remodeling complex to the beta-globin promoter in erythroid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 22 ·1999-10-26 ·Pages 12311-5

Lee CH, Murphy MR, Lee JS, Chung JH

Abstract

Chromatin remodeling complexes such as the SWI/SNF complex make DNA accessible to transcription factors by disrupting nucleosomes. However, it is not known how such complexes are targeted to the promoter. For example, a SWI/SNF1-like chromatin remodeling complex erythroid Krüppel-like factor (EKLF) coactivator-remodeling complex 1 (E-RC1) disrupts the nucleosomes over the human beta-globin promoter in an EKLF-dependent manner. However, it is not known whether E-RC1 is targeted specifically to the beta-globin promoter or whether E-RC1 is randomly targeted, but its activity is evident only at the beta-globin promoter. Because E-RC1 cannot remodel chromatin over the beta-globin promoter without EKLF in vitro, it has been proposed that SWI/SNF1-like complexes such as E-RC1 are targeted specifically to the promoter by selectively interacting with promoter-associated transcription factors such as EKLF. In this report, we test this hypothesis in the cellular context by using the ProteIN POsition Identification with Nuclease Tail (PIN*POINT) assay. We find that the Brahma-related gene (BRG) 1 and BRG1-associated factor (BAF) 170 subunits of E-RC1 are both recruited near the transcription initiation site of the beta-globin promoter. On transiently transfected templates, both the locus control region and the EKLF-binding site are important for their recruitment to the beta-globin promoter in mouse erythroleukemia cells. When the beta-globin promoter was linked to the cytomegalovirus enhancer, the E-RC1 complex was not recruited, suggesting that recruitment of the E-RC1 complex is not a general property of enhancers.

MeSH Terms
Base Sequence Chromatin/chemistry,metabolism DNA Primers Erythrocytes/metabolism Globins/genetics Humans Promoter Regions, Genetic Transcription Factors/metabolism
Chemicals
Chromatin DNA Primers Transcription Factors Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee C H
Molecular Hematology Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1654, USA.
Murphy M R
Lee J S
Chung J H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.22.12311
Published
1999-10-26
Pages
12311-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22913
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.96.22.12311 PMC Full Text View journal details
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