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PMID: 3038527 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The activation antigen BLAST-2, when shed, is an autocrine BCGF for normal and transformed B cells.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1637-42

Swendeman S, Thorley-Lawson DA

Abstract

A shed form of the membrane-bound B cell activation marker, BLAST-2 (Epstein-Barr virus cell surface, CD 23) was immune-affinity purified from Epstein-Barr virus-transformed lymphoblast conditioned medium. SDS-PAGE analysis revealed a complex of two polypeptides, mol. wts 25,000 and 12,000, here termed s-BLAST-2. We show that this complex, when purified to homogeneity, can act as a growth factor for EBV-infected B lymphoblasts and normal receptor-stimulated B cell blasts. It has no effect on resting B or T cells. These data suggest that the BLAST-2 antigen has a role in autocrine B cell growth. Additionally, this complex is a co-mitogen for PHA-stimulated murine thymocytes, a property of interleukin-1.

MeSH Terms
Antigens, Differentiation, B-Lymphocyte Antigens, Surface/immunology,isolation & purification B-Lymphocytes/immunology Cell Transformation, Viral Cells, Cultured Growth Substances/immunology Herpesvirus 4, Human/immunology Humans Interleukin-4 Lymphocyte Activation Lymphokines/immunology Molecular Weight Structure-Activity Relationship
Chemicals
Antigens, Differentiation, B-Lymphocyte Antigens, Surface Growth Substances Lymphokines Interleukin-4
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Swendeman S
Thorley-Lawson D A
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-06-00
Pages
1637-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553536
Subset
IM
Grants
NIAID NIH HHS · AI 00549 · United States
NIAID NIH HHS · AI 15310 · United States
NCI NIH HHS · CA 28737 · United States
External Links
PubMed source PMC Full Text View journal details
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