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PMID: 10880450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins.

The EMBO journal ·Vol. 19 ·No. 13 ·2000-07-03 ·Pages 3377-87

Poot RA, Dellaire G, Hülsmann BB, Grimaldi MA, Corona DF, Becker PB, Bickmore WA, Varga-Weisz PD

Abstract

Chromatin remodelling complexes containing the nucleosome-dependent ATPase ISWI were first isolated from Drosophila embryos (NURF, CHRAC and ACF). ISWI was the only common component reported in these complexes. Our purification of human CHRAC (HuCHRAC) shows that ISWI chromatin remodelling complexes can have a conserved subunit composition in completely different cell types, suggesting a conserved function of ISWI. We show that the human homologues of two novel putative histone-fold proteins in Drosophila CHRAC are present in HuCHRAC. The two human histone-fold proteins form a stable complex that binds naked DNA but not nucleosomes. HuCHRAC also contains human ACF1 (hACF1), the homologue of Acf1, a subunit of Drosophila ACF. The N-terminus of mouse ACF1 was reported as a heterochromatin-targeting domain. hACF1 is a member of a family of proteins with a related domain structure that all may target heterochromatin. We discuss a possible function for HuCHRAC in heterochromatin dynamics. HuCHRAC does not contain topoisomerase II, which was reported earlier as a subunit of Drosophila CHRAC.

MeSH Terms
Adenosine Triphosphatases/chemistry,metabolism Amino Acid Sequence Animals Chromosomal Proteins, Non-Histone DNA Polymerase III DNA-Binding Proteins/chemistry,metabolism Drosophila Drosophila Proteins HeLa Cells Histones/metabolism Humans Mice Molecular Sequence Data Nucleoproteins/chemistry,metabolism Nucleosomes/metabolism Protein Binding Protein Folding Recombinant Proteins/chemistry,metabolism Sequence Homology, Amino Acid Transcription Factors/chemistry,metabolism
Chemicals
Acf protein, Drosophila BAZ1A protein, human CHRAC1 protein, human Chromosomal Proteins, Non-Histone DNA-Binding Proteins Drosophila Proteins Histones ISWI protein Nucleoproteins Nucleosomes POLE3 protein, human Recombinant Proteins Transcription Factors DNA Polymerase III Adenosine Triphosphatases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Poot R A
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 OTL, MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
Dellaire G
Hülsmann B B
Grimaldi M A
Corona D F
Becker P B
Bickmore W A
Varga-Weisz P D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.13.3377
Published
2000-07-03
Pages
3377-87
Language
English
Region
England
NLM ID
8208664
PMCID
PMC313940
Subset
IM
Databases
GENBANK
AF213467, AF226076, AF226077
External Links
PubMed source DOI: 10.1093/emboj/19.13.3377 PMC Full Text View journal details
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