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PMID: 10090725 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae.

Genes & development ·Vol. 13 ·No. 6 ·1999-03-15 ·Pages 686-97

Tsukiyama T, Palmer J, Landel CC, Shiloach J, Wu C

Abstract

We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae, ISW1 and ISW2, which are highly related to Drosophila ISWI, encoding the putative ATPase subunit of three ATP-dependent chromatin remodeling factors. Purification of ISW1p reveals a four-subunit complex with nucleosome-stimulated ATPase activity, as well as ATP-dependent nucleosome disruption and spacing activities. Purification of ISW2p reveals a two-subunit complex also with nucleosome-stimulated ATPase and ATP-dependent nucleosome spacing activities but no detectable nucleosome disruption activity. Null mutations of ISW1, ISW2, and CHD1 genes cause synthetic lethality in various stress conditions in yeast cells, revealing the first in vivo functions of the ISWI subfamily of chromatin-remodeling complexes and demonstrating their genetic interactions. A single point mutation within the ATPase domain of both ISW1p and ISW2p inactivated all ATP-dependent biochemical activities of the complexes, as well as the ability of the genes to rescue the mutant phenotypes. This demonstrates that the ATP-dependent chromatin-remodeling activities are essential for the in vivo functions of both ISW1 and ISW2 complexes.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenosine Triphosphate/metabolism Chromatin/genetics Fungal Proteins/genetics,metabolism Genes, Fungal Nucleosomes/enzymology Point Mutation Saccharomyces cerevisiae/enzymology,genetics,metabolism
Chemicals
Chromatin Fungal Proteins Nucleosomes Adenosine Triphosphate Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tsukiyama T
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. ttsukiya@fhcrc.org
Palmer J
Landel C C
Shiloach J
Wu C
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.6.686
Email
ttsukiya@fhcrc.org
Published
1999-03-15
Pages
686-97
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316555
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.13.6.686 PMC Full Text View journal details
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