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PMID: 9256430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromatin assembly in a yeast whole-cell extract.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 17 ·1997-08-19 ·Pages 9034-9

Schultz MC, Hockman DJ, Harkness TA, Garinther WI, Altheim BA

Abstract

A simple in vitro system that supports chromatin assembly was developed for Saccharomyces cerevisiae. The assembly reaction is ATP-dependent, uses soluble histones and assembly factors, and generates physiologically spaced nucleosomes. We analyze the pathway of histone recruitment into nucleosomes, using this system in combination with genetic methods for the manipulation of yeast. This analysis supports the model of sequential recruitment of H3/H4 tetramers and H2A/H2B dimers into nucleosomes. Using a similar approach, we show that DNA ligase I can play an important role in template repair during assembly. These studies demonstrate the utility of this system for the combined biochemical and genetic analysis of chromatin assembly in yeast.

MeSH Terms
Chromatin/chemistry,genetics Nucleosomes/chemistry,genetics Saccharomyces cerevisiae/genetics
Chemicals
Chromatin Nucleosomes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schultz M C
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7. michael.schultz@ualberta.ca
Hockman D J
Harkness T A
Garinther W I
Altheim B A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.17.9034
Email
michael.schultz@ualberta.ca
Published
1997-08-19
Pages
9034-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23016
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.17.9034 PMC Full Text View journal details
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