Home LiteratureArticle Details
PMID: 1992452 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific initiation by RNA polymerase I in a whole-cell extract from yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 3 ·1991-02-01 ·Pages 1004-8

Schultz MC, Choe SY, Reeder RH

Abstract

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.

MeSH Terms
Base Sequence Cytosol/metabolism Genes, Fungal Molecular Sequence Data Oligonucleotide Probes Plasmids RNA Polymerase I/metabolism RNA Precursors/metabolism RNA, Ribosomal/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic
Chemicals
Oligonucleotide Probes RNA Precursors RNA, Ribosomal RNA Polymerase I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schultz M C
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Choe S Y
Reeder R H
References (28)
28 references, click to expand
  1. The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Jun 25;8(12):2679-89 PMID: 6159579
  2. The primary transcript of the ribosomal repeating unit in yeast.
    Nucleic Acids Res. 1979 Jan;6(1):27-39 PMID: 106368
  3. Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.
    J Biol Chem. 1982 Jul 25;257(14):8432-41 PMID: 7045122
  4. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.
    Nucleic Acids Res. 1982 Dec 20;10(24):8127-43 PMID: 6298710
  5. Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
    Cell. 1984 May;37(1):285-9 PMID: 6722873
  6. Transcription of an artificial ribosomal RNA gene in yeast.
    EMBO J. 1984 Jun;3(6):1377-82 PMID: 6086321
  7. The major promoter element of rRNA transcription in yeast lies 2 kb upstream.
    Cell. 1984 Dec;39(3 Pt 2):663-73 PMID: 6096018
  8. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  9. Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3391-4 PMID: 3517858
  10. Transcription of cloned eukaryotic ribosomal RNA genes.
    Annu Rev Biochem. 1986;55:801-30 PMID: 3527052
  11. A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter.
    Cell. 1986 Dec 26;47(6):913-20 PMID: 3779846
  12. Two forms of RPO41-dependent RNA polymerase. Regulation of the RNA polymerase by glucose repression may control yeast mitochondrial gene expression.
    J Biol Chem. 1988 Sep 5;263(25):12346-51 PMID: 3045116
  13. Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):486-90 PMID: 2643115
  14. Synthesis of ribosomes in Saccharomyces cerevisiae.
    Microbiol Rev. 1989 Jun;53(2):256-71 PMID: 2666845
  15. Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Aug;9(8):3464-72 PMID: 2677675
  16. Tagetitoxin: a new inhibitor of eukaryotic transcription by RNA polymerase III.
    J Biol Chem. 1990 Jan 5;265(1):499-505 PMID: 2403565
  17. Linker scanning of the yeast RNA polymerase I promoter.
    Nucleic Acids Res. 1989 Dec 11;17(23):9661-78 PMID: 2690005
  18. Specific transcription of Saccharomyces cerevisiae 35 S rDNA by RNA polymerase I in vitro.
    J Biol Chem. 1990 May 5;265(13):7596-603 PMID: 2185253
  19. Accurate initiation by RNA polymerase II in a whole cell extract from Saccharomyces cerevisiae.
    J Biol Chem. 1990 Jun 5;265(16):8979-82 PMID: 2188968
  20. An RNA polymerase I termination site can stimulate the adjacent ribosomal gene promoter by two distinct mechanisms in Xenopus laevis.
    Genes Dev. 1990 Jul;4(7):1240-51 PMID: 2210376
  21. Accurately initiated, enhancer-dependent transcription by RNA polymerase I in yeast extracts.
    J Biol Chem. 1990 Oct 25;265(30):18091-4 PMID: 2211685
  22. Transcription in yeast: separation and properties of multiple FNA polymerases.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702-6 PMID: 4558656
  23. Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization.
    J Biol Chem. 1974 Jan 10;249(1):241-8 PMID: 4855626
  24. Molecular structure of yeast RNA polymerase III: demonstration of the tripartite transcriptive system in lower eukaryotes.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1024-8 PMID: 772675
  25. Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1029-33 PMID: 772676
  26. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  27. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
    J Biol Chem. 1977 Nov 25;252(22):8118-25 PMID: 334774
  28. The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.
    Nucleic Acids Res. 1980 Nov 11;8(21):4919-26 PMID: 7003545
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.3.1004
Published
1991-02-01
Pages
1004-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50943
Subset
IM
Grants
NIGMS NIH HHS · GM26624 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.3.1004 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2