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PMID: 10864045 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

RNA secondary structures of the bacteriophage phi6 packaging regions.

RNA (New York, N.Y.) ·Vol. 6 ·No. 6 ·2000-06-00 ·Pages 880-9

Pirttimaa MJ, Bamford DH

Abstract

Bacteriophage phi6 genome consists of three segments of double-stranded RNA. During maturation, single-stranded copies of these segments are packaged into preformed polymerase complex particles. Only phi6 RNA is packaged, and each particle contains only one copy of each segment. An in vitro packaging and replication assay has been developed for phi6, and the packaging signals (pac sites) have been mapped to the 5' ends of the RNA segments. In this study, we propose secondary structure models for the pac sites of phi6 single-stranded RNA segments. Our models accommodate data from structure-specific chemical modifications, free energy minimizations, and phylogenetic comparisons. Previously reported pac site deletion studies are also discussed. Each pac site possesses a unique architecture, that, however, contains common structural elements.

MeSH Terms
Bacteriophage phi 6/chemistry,genetics Base Sequence Binding Sites/genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Viral/chemistry,genetics Reproducibility of Results Sequence Deletion Virus Assembly/genetics
Chemicals
RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pirttimaa M J
Institute of Biotechnology and Department of Biosciences, University of Helsinki, Finland.
Bamford D H
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838200992598
Published
2000-06-00
Pages
880-9
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369964
Subset
IM
External Links
PubMed source DOI: 10.1017/s1355838200992598 PMC Full Text View journal details
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