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PMID: 3754015 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the small double-stranded RNA segment of bacteriophage phi 6: novel mechanism of natural translational control.

Journal of virology ·Vol. 58 ·No. 1 ·1986-04-00 ·Pages 142-51

McGraw T, Mindich L, Frangione B

Abstract

The lipid-containing bacteriophage phi 6 has a genome composed of three segments of double-stranded RNA. We determined the nucleotide sequence of a cDNA copy of the smallest RNA segment. The coding sequences of the four proteins on this segment were identified. These sequences were clustered. Three of the genes had overlapping initiation-termination codons. All noncoding sequences were at the ends of the molecule. The genes of the small double-stranded RNA segment comprised two translational polarity groups. We propose that the translational coupling is the result of an inability of ribosomes to bind independently to two of the four genes. Translation of these genes occurred when ribosomes were delivered to them by translation of an upstream gene.

MeSH Terms
Bacteriophages/genetics Base Sequence Capsid/genetics Chromosome Mapping Codon DNA/genetics Gene Expression Regulation Genes, Viral Morphogenesis Muramidase/genetics Protein Biosynthesis RNA, Double-Stranded/genetics Viral Core Proteins/genetics Viral Envelope Proteins/genetics Viral Proteins/genetics
Chemicals
Codon RNA, Double-Stranded Viral Core Proteins Viral Envelope Proteins Viral Proteins DNA Muramidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McGraw T
Mindich L
Frangione B
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52 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.1.142-151.1986
Published
1986-04-00
Pages
142-51
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252886
Subset
IM
Grants
NIAID NIH HHS · 5T32-AI-07180 · United States
NIGMS NIH HHS · GM-31709 · United States
Databases
GENBANK
M12921
External Links
PubMed source DOI: 10.1128/JVI.58.1.142-151.1986 PMC Full Text View journal details
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