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PMID: 9108107 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stoichiometric packaging of the three genomic segments of double-stranded RNA bacteriophage phi6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 8 ·1997-04-15 ·Pages 4074-9

Qiao X, Qiao J, Mindich L

Abstract

A model that explains the stoichiometric packaging of the chromosomes of phi6, a bacteriophage with a genome of three unique double-stranded RNA segments, is proposed and supported. Ordered switches in packaging specificity and RNA synthesis are determined by the amount of RNA within the procapsid. The plus strand of segment S binds to one of several sites on the outside of the empty procapsid. The RNA enters and the procapsid expands so that the S sites are lost and M sites appear. Packaging of segment M results in the loss of the M sites and the appearance of the L sites. Packaging of L readies the particle for minus-strand synthesis. If any of the segments is less than normal size, packaging of that class of segments continues until the normal content of RNA for that segment is packaged and the binding sites then change.

MeSH Terms
Bacteriophages/genetics Genome, Viral Nucleic Acid Conformation RNA, Double-Stranded RNA, Viral
Chemicals
RNA, Double-Stranded RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Qiao X
Department of Microbiology, The Public Health Research Institute, New York, NY 10016, USA.
Qiao J
Mindich L
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.8.4074
Published
1997-04-15
Pages
4074-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20570
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034352 · United States
NIGMS NIH HHS · GM34352 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.8.4074 PMC Full Text View journal details
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