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PMID: 10856246 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

sigma factor selectivity of Escherichia coli RNA polymerase: role for CRP, IHF and lrp transcription factors.

The EMBO journal ·Vol. 19 ·No. 12 ·2000-06-15 ·Pages 3028-37

Colland F, Barth M, Hengge-Aronis R, Kolb A

Abstract

osmY is a stationary phase-induced and osmotically regulated gene in Escherichia coli that requires the stationary phase RNA polymerase (Esigma(S)) for in vivo expression. We show here that the major RNA polymerase, Esigma(70), also transcribes osmY in vitro and, depending on genetic background, even in vivo. The cAMP receptor protein (CRP) bound to cAMP, the leucine-responsive regulatory protein (Lrp) and the integration host factor (IHF) inhibit transcription initiation at the osmY promoter. The binding site for CRP is centred at -12.5 from the transcription start site, whereas Lrp covers the whole promoter region. The site for IHF maps in the -90 region. By mobility shift assay, permanganate reactivity and in vitro transcription experiments, we show that repression is much stronger with Esigma(70) than with Esigma(S) holoenzyme. We conclude that CRP, Lrp and IHF inhibit open complex formation more efficiently with Esigma(70) than with Esigma(S). This different ability of the two holoenzymes to interact productively with promoters once assembled in complex nucleoprotein structures may be a crucial factor in generating sigma(S) selectivity in vivo.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Binding Sites Carrier Proteins/genetics Cyclic AMP Receptor Protein/metabolism DNA-Binding Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Integration Host Factors Leucine-Responsive Regulatory Protein Molecular Sequence Data Periplasmic Binding Proteins Promoter Regions, Genetic Protein Binding Sigma Factor/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Cyclic AMP Receptor Protein DNA-Binding Proteins Escherichia coli Proteins Integration Host Factors Lrp protein, E coli Periplasmic Binding Proteins Sigma Factor Transcription Factors sigma factor KatF protein, Bacteria Leucine-Responsive Regulatory Protein osmY protein, E coli RNA polymerase sigma 70 DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Colland F
Institut Pasteur, Unité de Physicochimie des Macromolécules Biologiques (URA 1773 du CNRS), 75724 Paris, Cedex 15, France.
Barth M
Hengge-Aronis R
Kolb A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.12.3028
Published
2000-06-15
Pages
3028-37
Language
English
Region
England
NLM ID
8208664
PMCID
PMC203352
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.12.3028 PMC Full Text View journal details
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