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PMID: 9560209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A stationary phase protein in Escherichia coli with binding activity to the major sigma subunit of RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 9 ·1998-04-28 ·Pages 4953-8

Jishage M, Ishihama A

Abstract

Switching of the transcription pattern in Escherichia coli during the growth transition from exponential to stationary phase is accompanied by the replacement of the RNA polymerase-associated sigma70 subunit (sigmaD) with sigma38 (sigmaS). A fraction of the sigma70 subunit in stationary phase cell extracts was found to exist as a complex with a novel protein, designated Rsd (Regulator of sigma D). The intracellular level of Rsd starts to increase during the transition from growing to stationary phase. The rsd gene was identified at 90 min on the E. coli chromosome. Overexpressed and purified Rsd protein formed complexes in vitro with sigma70 but not with other sigma subunits, sigmaN, sigmaS, sigmaH, sigmaF, and sigmaE. Analysis of proteolytic fragments of sigma70 indicated that Rsd binds at or downstream of region 4, the promoter -35 recognition domain. The isolated Rsd inhibited transcription in vitro to various extents depending on the promoters used. We propose that Rsd is a stationary phase E. coli protein with regulatory activity of the sigma70 function.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/metabolism Cloning, Molecular Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Molecular Weight Promoter Regions, Genetic Protein Binding Repressor Proteins/metabolism Sigma Factor/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Escherichia coli Proteins Repressor Proteins Rsd protein, E coli Sigma Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jishage M
Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan.
Ishihama A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.9.4953
Published
1998-04-28
Pages
4953-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20194
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.9.4953 PMC Full Text View journal details
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