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PMID: 2690013 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.

Nucleic acids research ·Vol. 17 ·No. 23 ·1989-12-11 ·Pages 9979-91

Mulvey MR, Loewen PC

Abstract

The katF gene of Escherichia coli has been sequenced revealing a 1086 base pair open reading frame from which the sequence of a 362 amino acid protein has been deduced. The direction of transcription of katF was confirmed by expression of the gene cloned in both directions behind a T7 promoter. The KatF protein expressed in vitro migrates with an apparent size of 42 kDa. Comparison of the katF sequence to the sequence of rpoD, which encodes the sigma subunit of RNA polymerase, revealed a 181 bp region with 65% homology and a 38 bp segment that was 87% homologous. A 62 amino acid region of the predicted KatF protein sequence was found to be 85% homologous to the corresponding sequence of sigma 70, including a segment implicated in core polymerase binding. Homology was also observed with the heat shock regulatory protein encoded by htpR.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Sigma Factor/genetics Transcription Factors/genetics
Chemicals
Sigma Factor Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mulvey M R
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
Loewen P C
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.23.9979
Published
1989-12-11
Pages
9979-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335226
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.23.9979 PMC Full Text View journal details
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