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PMID: 6094482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 668-75

Loewen PC, Triggs BL

Abstract

A class of catalase-deficient mutants that was unlinked to katE was localized between mutS and cys at 59.0 min on the Escherichia coli genome. This locus was named katF. Transposon Tn10 insertions were isolated that mapped in both katE and katF loci. The catalase species present in katE+ and katF+ recombinants was found to be different from the main catalase activities, HPI and HPII, in several respects. It did not have an associated peroxidase activity; it was electrophoretically slower on native polyacrylamide gels; it eluted from DEAE-Sephadex A50 at a higher salt concentration; its Km for H2O2 was 30.9 mM as compared with 3.7 mM for HPI and HPII; its synthesis was not induced by ascorbate; and it did not cross react with HPI-HPII antisera. This new catalase was labeled HPIII.

MeSH Terms
Ascorbic Acid/pharmacology Catalase/genetics,metabolism Chromosome Mapping DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genetic Markers Genotype Immunodiffusion Kinetics Species Specificity
Chemicals
DNA Transposable Elements Genetic Markers Catalase DNA Restriction Enzymes Ascorbic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Loewen P C
Triggs B L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.668-675.1984
Published
1984-11-00
Pages
668-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214787
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.2.668-675.1984 PMC Full Text View journal details
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