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PMID: 8253679 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.

Journal of bacteriology ·Vol. 175 ·No. 24 ·1993-12-00 ·Pages 7910-7

Lange R, Barth M, Hengge-Aronis R

Abstract

osmY (csi-5) is a representative of a large group of sigma s-dependent genes in Escherichia coli that exhibit both stationary-phase induction and osmotic regulation. A chromosomal transcriptional lacZ fusion (csi-5::lacZ) was used to study the regulation of osmY. We show here that in addition to sigma s, the global regulators Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex (cAMP-CRP), and integration host factor (IHF) are involved in the control of osmY. All three regulators negatively modulate the expression of osmY, and they act independently from sigma s. Stationary-phase induction of osmY in minimal medium can be explained by stimulation by sigma s combined with a relief of Lrp repression. Stationary-phase induction of osmY in rich medium is mediated by the combined action of sigma s, Lrp, cAMP-CRP, and IHF, with the latter three proteins acting as transition state regulators. The transcriptional start site of osmY was determined and revealed an mRNA with an unusual long nontranslated leader of 244 nucleotides. The regulatory region is characterized by a sigma 70-like -10 promoter region and contains potential binding sites for Lrp, CRP, and IHF. Whereas sigma s, Lrp, CRP, and IHF are clearly involved in stationary-phase induction, none of these regulators is essential for osmotic regulation of osmY.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,metabolism Base Sequence Binding Sites Carrier Proteins/biosynthesis Cyclic AMP/metabolism Cyclic AMP Receptor Protein/metabolism DNA Primers DNA, Bacterial/chemistry,metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Genotype Heat-Shock Proteins/metabolism Integration Host Factors Kinetics Molecular Sequence Data Mutagenesis Open Reading Frames Osmolar Concentration Periplasmic Binding Proteins Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Sigma Factor/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Cyclic AMP Receptor Protein DNA Primers DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Heat-Shock Proteins Integration Host Factors Periplasmic Binding Proteins SigB protein, Bacteria Sigma Factor Transcription Factors osmY protein, E coli Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lange R
Department of Biology, University of Konstanz, Germany.
Barth M
Hengge-Aronis R
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.24.7910-7917.1993
Published
1993-12-00
Pages
7910-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206969
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.24.7910-7917.1993 PMC Full Text View journal details
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