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PMID: 1658595 Published · ppublish English Journal Article

A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA)

Molecular & general genetics : MGG ·Vol. 229 ·No. 3 ·1991-10-00 ·Pages 341-52

Dassa J, Fsihi H, Marck C, Dion M, Kieffer-Bontemps M, Boquet PL

Abstract

The Escherichia coli acid phosphatase gene appA is expressed in response to oxygen deprivation and is positively controlled by the product of appR (katF) which encodes a putative new sigma transcription-initiation factor. However, transcription of appA from its nearest promoter (P1) did not account for total pH 2.5 acid phosphatase expression and was not subject to regulation. The cloned region upstream of appA was extended and analyzed by insertions of transposon TnphoA and by fusions with lacZ. It contains two new genes, appC and appB, which both encode extracytoplasmic proteins. appC and appB are expressed from a promoter (P2) lying just upstream of appC. Both genes are regulated by oxygen, as is appA, and by appR gene product exactly as previously shown for appA. Analysis of the nucleotide sequence and of the origins of transcription have confirmed that the P2-appC-appB- (ORFX)-P1-appA region is organized on the chromosome as an operon transcribed clockwise from P2 and that P1 is a minor promoter for appA alone. Genes appC and appB encode proteins of Mr 58,133 and 42,377, respectively, which have the characteristics of integral membrane proteins. The deduced amino acid sequences of appC and appB show 60% and 57% homology, respectively, with subunits I and II of the E. coli cytochrome d oxidase (encoded by genes cydA and cydB). The notion that the AppC and AppB proteins constitute a new cytochrome oxidase or a new oxygen-detoxifying system is supported by the observation of enhanced sensitivity to oxygen of mutants lacking all three genes, cyo (cytochrome o oxidase), cyd (cytochrome d oxidase) and appB, compared to that of cyo cyd double mutants.

MeSH Terms
Acid Phosphatase/genetics,metabolism Amino Acid Sequence Bacterial Outer Membrane Proteins Bacterial Proteins/genetics,metabolism Base Sequence DNA Transposable Elements DNA, Bacterial Electron Transport Complex IV/genetics,metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Hydrogen-Ion Concentration Molecular Sequence Data Mutagenesis Operon Oxidoreductases Oxygen/physiology Promoter Regions, Genetic Restriction Mapping Sequence Alignment Transcription, Genetic Transduction, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA Transposable Elements DNA, Bacterial Escherichia coli Proteins CbdA protein, E coli cbdB protein, E coli Oxidoreductases Electron Transport Complex IV Acid Phosphatase Oxygen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dassa J
Service de Biochimie des Proteines, C.E. Saclay, Gif-sur-Yvette, France.
Fsihi H
Marck C
Dion M
Kieffer-Bontemps M
Boquet P L
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267454
Published
1991-10-00
Pages
341-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S55526, S55527, S55528, S55529, S55530, S55531, S55532, S63744, S63748, S63811
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/BF00267454
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