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PMID: 10542189 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription.

Journal of bacteriology ·Vol. 181 ·No. 21 ·1999-11-00 ·Pages 6836-9

Landini P, Busby SJ

Abstract

The Escherichia coli Ada protein activates sigma(70)-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA. During stationary phase, however, the methylated form of Ada shuts off expression of alkA; this repression is specific for sigma(S)-dependent transcription. Thus, at the alkA promoter, the Ada protein can act as both a positive and negative modulator of the adaptive response to alkylation damage, depending on the cell's physiological state.

MeSH Terms
Bacterial Proteins/genetics,metabolism DNA Glycosylases Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial N-Glycosyl Hydrolases/genetics,metabolism O(6)-Methylguanine-DNA Methyltransferase Regulon Sigma Factor/genetics,metabolism Transcription Factors Transcription, Genetic
Chemicals
AidB protein, E coli Bacterial Proteins Escherichia coli Proteins Sigma Factor Transcription Factors sigma factor KatF protein, Bacteria Ada protein, E coli O(6)-Methylguanine-DNA Methyltransferase DNA Glycosylases N-Glycosyl Hydrolases DNA-3-methyladenine glycosidase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Landini P
Swiss Federal Institute for Environmental Science and Technology (EAWAG), 8600 Dübendorf, Switzerland. landini@eawag.ch
Busby S J
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.21.6836-6839.1999
Email
landini@eawag.ch
Published
1999-11-00
Pages
6836-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94152
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.21.6836-6839.1999 PMC Full Text View journal details
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