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PMID: 10852879 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA.

Journal of bacteriology ·Vol. 182 ·No. 12 ·2000-06-00 ·Pages 3467-74

Barbosa TM, Levy SB

Abstract

In Escherichia coli, the MarA protein controls expression of multiple chromosomal genes affecting resistance to antibiotics and other environmental hazards. For a more-complete characterization of the mar regulon, duplicate macroarrays containing 4,290 open reading frames of the E. coli genome were hybridized to radiolabeled cDNA populations derived from mar-deleted and mar-expressing E. coli. Strains constitutively expressing MarA showed altered expression of more than 60 chromosomal genes: 76% showed increased expression and 24% showed decreased expression. Although some of the genes were already known to be MarA regulated, the majority were newly determined and belonged to a variety of functional groups. Some of the genes identified have been associated with iron transport and metabolism; other genes were previously known to be part of the soxRS regulon. Northern blot analysis of selected genes confirmed the results obtained with the macroarrays. The findings reveal that the mar locus mediates a global stress response involving one of the largest networks of genes described.

MeSH Terms
Bacterial Proteins/genetics,metabolism Blotting, Northern Chromosome Mapping Chromosomes, Bacterial/genetics DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genome, Bacterial Iron/metabolism Oligonucleotide Array Sequence Analysis Operon Regulon Trans-Activators Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins MarA protein, E coli Trans-Activators Transcription Factors SoxR protein, Bacteria SoxS protein, E coli Iron
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barbosa T M
Center for Adaptation Genetics and Drug Resistance and the Departments of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Levy S B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.12.3467-3474.2000
Published
2000-06-00
Pages
3467-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC101932
Subset
IM
Grants
NIGMS NIH HHS · GM51661 · United States
External Links
PubMed source DOI: 10.1128/JB.182.12.3467-3474.2000 PMC Full Text View journal details
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