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PMID: 9473050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.

Journal of bacteriology ·Vol. 180 ·No. 4 ·1998-02-00 ·Pages 938-44

Aono R, Tsukagoshi N, Yamamoto M

Abstract

Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that tolC is a member of the mar-sox regulon responsive to stress conditions. When a defective tolC gene was transferred to n-hexane- or cyclohexane-tolerant strains by P1 transduction, the organic solvent tolerance level was lowered dramatically to the decane-tolerant and nonane-sensitive level. The tolerance level was restored by transformation of the transductants with a wild-type tolC gene. Therefore, it is evident that TolC is essential for E. coli to maintain organic solvent tolerance.

MeSH Terms
Alkanes/pharmacology Anti-Bacterial Agents/pharmacology Bacterial Outer Membrane Proteins/biosynthesis,genetics Bacterial Proteins/genetics Biological Transport Cyclohexanes/pharmacology DNA-Binding Proteins/genetics Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Hexanes/pharmacology Hydrocarbons/pharmacology Membrane Transport Proteins Microbial Sensitivity Tests Mutation Regulon Repressor Proteins/genetics Solvents/pharmacology Trans-Activators Transcription Factors/genetics Transduction, Genetic
Chemicals
Alkanes Anti-Bacterial Agents Bacterial Outer Membrane Proteins Bacterial Proteins Cyclohexanes DNA-Binding Proteins Escherichia coli Proteins Hexanes Hydrocarbons MarA protein, E coli MarR protein, E coli Membrane Transport Proteins Repressor Proteins Rob protein, E coli Solvents Trans-Activators Transcription Factors tolC protein, E coli SoxS protein, E coli decane
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aono R
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. raono@bio.titech.ac.jp
Tsukagoshi N
Yamamoto M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.4.938-944.1998
Email
raono@bio.titech.ac.jp
Published
1998-02-00
Pages
938-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC106975
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.4.938-944.1998 PMC Full Text View journal details
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