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PMID: 9097440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli.

Applied and environmental microbiology ·Vol. 63 ·No. 4 ·1997-04-00 ·Pages 1428-33

Asako H, Nakajima H, Kobayashi K, Kobayashi M, Aono R

Abstract

We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ. Microbiol. 61:2302-2307, 1995). Most E. coli strains cannot grow in the presence of cyclohexane. We isolated the marRAB genes from a Kohara lambda phage clone and cyclohexane-tolerant mutant strain OST3408. We found a substitution of serine for arginine at position 73 in the coding region of marR of OST3408 and designated the gene marR08. Our genetic analysis revealed that marR08 is responsible for the cyclohexane-tolerant phenotype. We observed that the marA gene on high-copy-number plasmids increased the organic solvent tolerance of E. coli strains. Furthermore, exposure of E. coli cells to salicylate, which activates the mar regulon genes, also raised organic solvent tolerance. Overexpression of the marA, soxS, or robA gene increased resistance to numerous antibiotics but not to hydrophilic aminoglycosides.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/genetics DNA-Binding Proteins/genetics Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Solvents/pharmacology
Chemicals
Anti-Bacterial Agents Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins MarA protein, E coli Solvents
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Asako H
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Nakajima H
Kobayashi K
Kobayashi M
Aono R
References (28)
28 references, click to expand
  1. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.
    Gene. 1979 Oct;7(2):141-52 PMID: 389741
  2. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  3. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  4. Direction of travel of RecBC recombinase through bacteriophage lambda DNA.
    Genetics. 1986 Jun;113(2):215-27 PMID: 3013720
  5. Charomids: cosmid vectors for efficient cloning and mapping of large or small restriction fragments.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8664-8 PMID: 3022301
  6. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  7. marA locus causes decreased expression of OmpF porin in multiple-antibiotic-resistant (Mar) mutants of Escherichia coli.
    J Bacteriol. 1988 Dec;170(12):5416-22 PMID: 2848006
  8. marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1991 Sep;173(17):5532-8 PMID: 1715857
  9. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.
    J Bacteriol. 1993 Mar;175(5):1484-92 PMID: 8383113
  10. The XylS/AraC family of regulators.
    Nucleic Acids Res. 1993 Feb 25;21(4):807-10 PMID: 8451183
  11. Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1993 May;175(10):2888-94 PMID: 8491710
  12. Active efflux of chloramphenicol in susceptible Escherichia coli strains and in multiple-antibiotic-resistant (Mar) mutants.
    Antimicrob Agents Chemother. 1994 Mar;38(3):542-6 PMID: 8203852
  13. Dual regulation of inaA by the multiple antibiotic resistance (mar) and superoxide (soxRS) stress response systems of Escherichia coli.
    J Bacteriol. 1994 Oct;176(20):6262-9 PMID: 7928997
  14. Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.
    Appl Environ Microbiol. 1994 Dec;60(12):4624-6 PMID: 7811102
  15. Identification of new genes regulated by the marRAB operon in Escherichia coli.
    J Bacteriol. 1995 Feb;177(3):530-5 PMID: 7836283
  16. Activation of multiple antibiotic resistance and binding of stress-inducible promoters by Escherichia coli Rob protein.
    J Bacteriol. 1995 Apr;177(7):1655-61 PMID: 7896685
  17. A close correlation between improvement of organic solvent tolerance levels and alteration of resistance toward low levels of multiple antibiotics in Escherichia coli.
    Biosci Biotechnol Biochem. 1995 Feb;59(2):213-8 PMID: 7766020
  18. Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli.
    J Bacteriol. 1995 Jun;177(12):3414-9 PMID: 7768850
  19. Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences.
    Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5456-60 PMID: 7777530
  20. Overexpression of the robA gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli.
    Appl Environ Microbiol. 1995 Jun;61(6):2302-7 PMID: 7793951
  21. Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7617-21 PMID: 7644465
  22. Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli.
    Mol Microbiol. 1995 Apr;16(1):45-55 PMID: 7651136
  23. soxRS gene increased the level of organic solvent tolerance in Escherichia coli.
    Biosci Biotechnol Biochem. 1995 Jul;59(7):1323-5 PMID: 7670195
  24. Purification and regulatory properties of MarA protein, a transcriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters.
    J Bacteriol. 1995 Dec;177(24):7100-4 PMID: 8522515
  25. Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli.
    J Bacteriol. 1996 Apr;178(8):2216-23 PMID: 8636021
  26. Active efflux of toluene in a solvent-resistant bacterium.
    J Bacteriol. 1996 Oct;178(20):6056-8 PMID: 8830706
  27. Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1994 Jan;176(1):143-8 PMID: 8282690
  28. P1 transduction map spanning the replication terminus of Escherichia coli K12.
    Mol Gen Genet. 1981;184(2):208-12 PMID: 6276692
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.63.4.1428-1433.1997
Published
1997-04-00
Pages
1428-33
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC168437
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.63.4.1428-1433.1997 PMC Full Text View journal details
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