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PMID: 9324261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of the acrAB locus in organic solvent tolerance mediated by expression of marA, soxS, or robA in Escherichia coli.

Journal of bacteriology ·Vol. 179 ·No. 19 ·1997-10-00 ·Pages 6122-6

White DG, Goldman JD, Demple B, Levy SB

Abstract

Escherichia coli K-12 strains are normally tolerant to n-hexane and susceptible to cyclohexane. Constitutive expression of marA of the multiple antibiotic resistance (mar) locus or of the soxS or robA gene product produced tolerance to cyclohexane. Inactivation of the mar locus or the robA locus, but not the soxRS locus, increased organic solvent susceptibility in the wild type and Mar mutants (to both n-hexane and cyclohexane). The organic solvent hypersusceptibility is a newly described phenotype for a robA-inactivated strain. Multicopy expression of mar, soxS, or robA induced cyclohexane tolerance in strains with a deleted or inactivated chromosomal mar, soxRS, or robA locus; thus, each transcriptional activator acts independently of the others. However, in a strain with 39 kb of chromosomal DNA, including the mar locus, deleted, only the multicopy complete mar locus, consisting of its two operons, produced cyclohexane tolerance. Deletion of acrAB from either wild-type E. coli K-12 or a Mar mutant resulted in loss of tolerance to both n-hexane and cyclohexane. Organic solvent tolerance mediated by mar, soxS, or robA was not restored in strains with acrAB deleted. These findings strongly suggest that active efflux specified by the acrAB locus is linked to intrinsic organic solvent tolerance and to tolerance mediated by the marA, soxS, or robA gene product in E. coli.

MeSH Terms
Bacterial Proteins/genetics Carrier Proteins Cyclohexanes/pharmacology DNA-Binding Proteins/genetics Drug Resistance, Microbial/genetics Drug Tolerance/genetics Escherichia coli/drug effects,genetics,growth & development Escherichia coli Proteins Gene Expression Hexanes/pharmacology Lipoproteins/genetics Membrane Proteins/genetics Membrane Transport Proteins Multidrug Resistance-Associated Proteins Operon Solvents/pharmacology Trans-Activators Transcription Factors/genetics
Chemicals
AcrA protein, E coli AcrB protein, E coli Bacterial Proteins Carrier Proteins Cyclohexanes DNA-Binding Proteins Escherichia coli Proteins Hexanes Lipoproteins MarA protein, E coli Membrane Proteins Membrane Transport Proteins Multidrug Resistance-Associated Proteins Rob protein, E coli Solvents Trans-Activators Transcription Factors SoxS protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
White D G
Center for Adaptation Genetics and Drug Resistance and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Goldman J D
Demple B
Levy S B
References (35)
35 references, click to expand
  1. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.
    J Bacteriol. 1993 Mar;175(5):1484-92 PMID: 8383113
  2. Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene.
    J Bacteriol. 1992 Oct;174(19):6054-60 PMID: 1400156
  3. Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1993 May;175(10):2888-94 PMID: 8491710
  4. Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1994 Jan;176(1):143-8 PMID: 8282690
  5. Active efflux of chloramphenicol in susceptible Escherichia coli strains and in multiple-antibiotic-resistant (Mar) mutants.
    Antimicrob Agents Chemother. 1994 Mar;38(3):542-6 PMID: 8203852
  6. SoxS, an activator of superoxide stress genes in Escherichia coli. Purification and interaction with DNA.
    J Biol Chem. 1994 Jul 15;269(28):18371-7 PMID: 8034583
  7. Dual regulation of inaA by the multiple antibiotic resistance (mar) and superoxide (soxRS) stress response systems of Escherichia coli.
    J Bacteriol. 1994 Oct;176(20):6262-9 PMID: 7928997
  8. Genetic relationship between soxRS and mar loci in promoting multiple antibiotic resistance in Escherichia coli.
    Antimicrob Agents Chemother. 1994 Aug;38(8):1773-9 PMID: 7986007
  9. Purification of a MalE-SoxS fusion protein and identification of the control sites of Escherichia coli superoxide-inducible genes.
    Mol Microbiol. 1994 Nov;14(4):669-79 PMID: 7891555
  10. Activation of multiple antibiotic resistance and binding of stress-inducible promoters by Escherichia coli Rob protein.
    J Bacteriol. 1995 Apr;177(7):1655-61 PMID: 7896685
  11. A close correlation between improvement of organic solvent tolerance levels and alteration of resistance toward low levels of multiple antibiotics in Escherichia coli.
    Biosci Biotechnol Biochem. 1995 Feb;59(2):213-8 PMID: 7766020
  12. Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli.
    J Bacteriol. 1995 Jun;177(12):3414-9 PMID: 7768850
  13. Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences.
    Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5456-60 PMID: 7777530
  14. Overexpression of the robA gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli.
    Appl Environ Microbiol. 1995 Jun;61(6):2302-7 PMID: 7793951
  15. Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7617-21 PMID: 7644465
  16. Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli.
    Mol Microbiol. 1995 Apr;16(1):45-55 PMID: 7651136
  17. soxRS gene increased the level of organic solvent tolerance in Escherichia coli.
    Biosci Biotechnol Biochem. 1995 Jul;59(7):1323-5 PMID: 7670195
  18. AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants.
    J Bacteriol. 1996 Jan;178(1):306-8 PMID: 8550435
  19. Transcriptional activation of promoters of the superoxide and multiple antibiotic resistance regulons by Rob, a binding protein of the Escherichia coli origin of chromosomal replication.
    J Bacteriol. 1996 May;178(9):2507-13 PMID: 8626315
  20. Multiple antibiotic resistance (mar) locus protects Escherichia coli from rapid cell killing by fluoroquinolones.
    Antimicrob Agents Chemother. 1996 May;40(5):1266-9 PMID: 8723480
  21. Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli.
    Antimicrob Agents Chemother. 1996 Jul;40(7):1695-8 PMID: 8807064
  22. The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals.
    Mol Microbiol. 1996 Jan;19(1):101-12 PMID: 8821940
  23. Active efflux of toluene in a solvent-resistant bacterium.
    J Bacteriol. 1996 Oct;178(20):6056-8 PMID: 8830706
  24. Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli.
    Appl Environ Microbiol. 1997 Apr;63(4):1428-33 PMID: 9097440
  25. Effects of toluene on Escherichia coli.
    J Bacteriol. 1965 Nov;90(5):1420-5 PMID: 5321488
  26. Effects of toluene and phenethyl alcohol on the ultrastructure of Escherichia coli.
    J Bacteriol. 1973 Jun;114(3):1359-61 PMID: 4576406
  27. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli.
    Biochim Biophys Acta. 1978 Jan 4;506(1):64-80 PMID: 413578
  28. Amplifiable resistance to tetracycline, chloramphenicol, and other antibiotics in Escherichia coli: involvement of a non-plasmid-determined efflux of tetracycline.
    J Bacteriol. 1983 Aug;155(2):531-40 PMID: 6348022
  29. marA locus causes decreased expression of OmpF porin in multiple-antibiotic-resistant (Mar) mutants of Escherichia coli.
    J Bacteriol. 1988 Dec;170(12):5416-22 PMID: 2848006
  30. Cross-resistance to fluoroquinolones in multiple-antibiotic-resistant (Mar) Escherichia coli selected by tetracycline or chloramphenicol: decreased drug accumulation associated with membrane changes in addition to OmpF reduction.
    Antimicrob Agents Chemother. 1989 Aug;33(8):1318-25 PMID: 2679373
  31. Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6181-5 PMID: 1696718
  32. Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli.
    J Bacteriol. 1991 May;173(9):2864-71 PMID: 1708380
  33. Activation of oxidative stress genes by mutations at the soxQ/cfxB/marA locus of Escherichia coli.
    J Bacteriol. 1991 Jul;173(14):4433-9 PMID: 1648558
  34. Molecular characterization of the soxRS genes of Escherichia coli: two genes control a superoxide stress regulon.
    Nucleic Acids Res. 1991 Aug 25;19(16):4479-84 PMID: 1653416
  35. A novel binding protein of the origin of the Escherichia coli chromosome.
    J Biol Chem. 1993 Mar 15;268(8):5365-70 PMID: 8449900
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.19.6122-6126.1997
Published
1997-10-00
Pages
6122-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179517
Subset
IM
Grants
NCI NIH HHS · CA3731 · United States
NIGMS NIH HHS · GM51661 · United States
NIAID NIH HHS · T32 AI-07329 · United States
External Links
PubMed source DOI: 10.1128/jb.179.19.6122-6126.1997 PMC Full Text View journal details
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