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PMID: 9293016 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12.

Applied and environmental microbiology ·Vol. 63 ·No. 9 ·1997-09-00 ·Pages 3637-42

Aono R, Kobayashi H

Abstract

In this study, we examined cell surface properties of mutants of Escherichia coli for which organic solvent tolerance levels were elevated. The cell surface of each mutant was less hydrophobic than that of the parent, probably due to an increase in lipopolysaccharide content. OmpF synthesis was repressed in the mutants. Organic solvent bound readily to viable E. coli cells in response to the polarity of the solvent. The mutants were bound less abundantly with the organic solvent than was the parent.

MeSH Terms

Bacterial Adhesion/genetics Bacterial Outer Membrane Proteins/biosynthesis,genetics Cell Membrane/metabolism Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics,metabolism Lipoproteins/metabolism Mutation Octanes/metabolism,toxicity Peptidoglycan/metabolism Solvents/chemistry,metabolism,toxicity

Chemicals

Bacterial Outer Membrane Proteins Lipoproteins Octanes Peptidoglycan Solvents octane

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Aono R

Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

Kobayashi H

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26 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.63.9.3637-3642.1997

Published

1997-09-00

Pages

3637-42

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC168671

Subset

External Links

PubMed source DOI: 10.1128/aem.63.9.3637-3642.1997 PMC Full Text View journal details

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Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12.

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