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PMID: 10440227 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Stress and the cell nucleus: dynamics of gene expression and structural reorganization.

Gene expression ·Vol. 7 ·No. 4-6 ·1999-00-00 ·Pages 261-70

Jolly C, Morimoto RI

Abstract

A growing number of experimental observations reveal that the cell nucleus is functionally compartmentalized yet organized to ensure a dynamic response to events that influence nuclear activities. The cellular and molecular response to physiological and environmental stress induces a rapid and transient change in gene expression associated with major changes in nuclear architecture that impacts on signals involved in cell growth. In this review, we will address the effects of stress on the functional compartmentation of the cell nucleus and the dynamic reorganization of nuclear structures and function.

MeSH Terms
Animals Cell Compartmentation Cell Nucleus/metabolism,physiology,ultrastructure Gene Expression Humans Models, Biological
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jolly C
Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA.
Morimoto R I
References (84)
84 references, click to expand
  1. Morphological study of the mammalian stress response: characterization of changes in cytoplasmic organelles, cytoskeleton, and nucleoli, and appearance of intranuclear actin filaments in rat fibroblasts after heat-shock treatment.
    J Cell Biol. 1985 Oct;101(4):1198-211 PMID: 3900086
  2. Transcriptional regulation in Drosophila during heat shock: a nuclear run-on analysis.
    Chromosoma. 1993 Mar;102(4):233-48 PMID: 8486075
  3. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins.
    Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6975-80 PMID: 8692929
  4. Heat shock selectively inhibits ribosomal RNA gene transcription and down-regulates E1BF/Ku in mouse lymphosarcoma cells.
    Biochem J. 1996 Aug 1;317 ( Pt 3):689-95 PMID: 8760351
  5. Chaperoning brain diseases.
    Nature. 1998 Mar 5;392(6671):23-4 PMID: 9510241
  6. Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport.
    Mol Biol Cell. 1996 Jan;7(1):173-92 PMID: 8741848
  7. Apoptosis, the heat shock response, hyperthermia, birth defects, disease and cancer. Where are the common links?
    Cell Stress Chaperones. 1998 Dec;3(4):213-20 PMID: 9880233
  8. Role of the major heat shock proteins as molecular chaperones.
    Annu Rev Cell Biol. 1993;9:601-34 PMID: 8280473
  9. Compartmentalization of eukaryotic gene expression: causes and effects.
    Cell. 1997 Oct 31;91(3):291-4 PMID: 9363936
  10. Regulation of translational initiation during cellular responses to stress.
    Prog Nucleic Acid Res Mol Biol. 1998;58:79-125 PMID: 9308364
  11. Identification of a nuclear protein matrix.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410-7 PMID: 4214419
  12. Green fluorescent protein as a marker for gene expression.
    Science. 1994 Feb 11;263(5148):802-5 PMID: 8303295
  13. The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.
    Mol Cell Biol. 1991 Nov;11(11):5624-30 PMID: 1922067
  14. The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping.
    Nucleic Acids Res. 1996 Jul 1;24(13):2535-42 PMID: 8692693
  15. Heterochromatin and gene regulation in Drosophila.
    Curr Opin Genet Dev. 1996 Apr;6(2):193-202 PMID: 8722176
  16. Compartmentalization of specific pre-mRNA metabolism: an emerging view.
    Hum Mol Genet. 1995;4 Spec No:1779-89 PMID: 8541878
  17. Alternative 5' splice site selection induced by heat shock.
    Mol Cell Biol. 1994 Jan;14(1):567-75 PMID: 8264624
  18. Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators.
    Genes Dev. 1998 Dec 15;12(24):3788-96 PMID: 9869631
  19. Activation of programmed cell death (apoptosis) by cisplatin, other anticancer drugs, toxins and hyperthermia.
    Biochem Pharmacol. 1990 Nov 15;40(10):2353-62 PMID: 2244936
  20. Thinking about a nuclear matrix.
    J Mol Biol. 1998 Mar 27;277(2):147-59 PMID: 9514762
  21. The nuclear matrix: a target for heat shock effects and a determinant for stress response.
    Crit Rev Eukaryot Gene Expr. 1997;7(4):343-60 PMID: 9607170
  22. Activation of heat shock factor 2 during hemin-induced differentiation of human erythroleukemia cells.
    Mol Cell Biol. 1992 Sep;12(9):4104-11 PMID: 1508207
  23. Induced heat shock mRNAs escape the nucleocytoplasmic transport block in adenovirus-infected HeLa cells.
    Mol Cell Biol. 1987 Dec;7(12):4505-12 PMID: 3437895
  24. Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export.
    EMBO J. 1996 Dec 2;15(23):6750-7 PMID: 8978700
  25. Contribution of growing RNA molecules to the nuclear transcripts foci observed by FISH.
    Exp Cell Res. 1998 Jan 10;238(1):299-304 PMID: 9457084
  26. The green fluorescent protein.
    Annu Rev Biochem. 1998;67:509-44 PMID: 9759496
  27. Active and inactive genes localize preferentially in the periphery of chromosome territories.
    J Cell Biol. 1996 Dec;135(5):1195-205 PMID: 8947544
  28. Repeated mild heat shock delays ageing in cultured human skin fibroblasts.
    Biochem Mol Biol Int. 1998 Jul;45(4):753-9 PMID: 9713698
  29. Identification of an interchromosomal compartment by polymerization of nuclear-targeted vimentin.
    J Cell Sci. 1998 May;111 ( Pt 9):1241-53 PMID: 9547300
  30. Hsp90 as a capacitor for morphological evolution.
    Nature. 1998 Nov 26;396(6709):336-42 PMID: 9845070
  31. The RNA polymerase II molecule at the 5' end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged.
    Cell. 1988 Sep 9;54(6):795-804 PMID: 3136931
  32. HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator.
    Mol Cell Biol. 1997 Jan;17(1):469-81 PMID: 8972228
  33. Nuclear matrix as a target for hyperthermic killing of cancer cells.
    Cell Stress Chaperones. 1998 Dec;3(4):245-55 PMID: 9880237
  34. Heat shock affects 5' splice site selection, cleavage and ligation of CAD pre-mRNA in hamster cells, but not its packaging in InRNP particles.
    Nucleic Acids Res. 1994 Aug 11;22(15):3084-91 PMID: 7915031
  35. Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4501-13 PMID: 6368558
  36. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
    Nature. 1990 Feb 1;343(6257):437-41 PMID: 2137203
  37. Poly(A) tail length of a heat shock protein RNA is increased by severe heat stress, but intron splicing is unaffected.
    Mol Gen Genet. 1993 Jun;239(3):323-33 PMID: 8391109
  38. A functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-mRNA splicing.
    J Cell Biol. 1997 Jan 13;136(1):5-18 PMID: 9008699
  39. Heat shock but not other stress inducers leads to the disruption of a sub-set of snRNPs and inhibition of in vitro splicing in HeLa cells.
    EMBO J. 1988 Nov;7(11):3509-18 PMID: 2974799
  40. Role of Hsp70 in regulation of stress-kinase JNK: implications in apoptosis and aging.
    FEBS Lett. 1998 Oct 30;438(1-2):1-4 PMID: 9821948
  41. In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7923-7 PMID: 8367444
  42. Tissue-specific alternative splicing of the Drosophila dopa decarboxylase gene is affected by heat shock.
    Mol Cell Biol. 1993 Aug;13(8):4549-55 PMID: 8336700
  43. Role of the human heat shock protein hsp70 in protection against stress-induced apoptosis.
    Mol Cell Biol. 1997 Sep;17(9):5317-27 PMID: 9271409
  44. Monoclonal antibody CC-3 recognizes phosphoproteins in interphase and mitotic cells.
    Exp Cell Res. 1991 Jul;195(1):145-53 PMID: 2055264
  45. Applications of the green fluorescent protein in cell biology and biotechnology.
    Nat Biotechnol. 1997 Oct;15(10):961-4 PMID: 9335045
  46. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  47. Synthesis of trypanosome hsp70 mRNA is resistant to disruption of trans-splicing by heat shock.
    J Biol Chem. 1989 May 5;264(13):7107-10 PMID: 2708359
  48. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.
    Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8253-7 PMID: 8710856
  49. RNA polymerase II pauses at the 5' end of the transcriptionally induced Drosophila hsp70 gene.
    Mol Cell Biol. 1991 Oct;11(10):5285-90 PMID: 1922045
  50. HSF1 transcription factor concentrates in nuclear foci during heat shock: relationship with transcription sites.
    J Cell Sci. 1997 Dec;110 ( Pt 23):2935-41 PMID: 9359877
  51. Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress.
    Mol Cell Biol. 1993 Mar;13(3):1392-407 PMID: 8441385
  52. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription.
    Nature. 1997 Jan 23;385(6614):357-61 PMID: 9002523
  53. Heat stress-induced life span extension in yeast.
    Exp Cell Res. 1998 Dec 15;245(2):379-88 PMID: 9851879
  54. Variation in sensitivity to heat shock during the cell-cycle of Chinese hamster cells in vitro.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(5):467-77 PMID: 5314347
  55. Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway.
    Mol Cell Biol. 1998 Sep;18(9):5091-8 PMID: 9710593
  56. Regulating genes by packaging domains: bits of heterochromatin in euchromatin?
    Trends Genet. 1993 Feb;9(2):35-7 PMID: 8456498
  57. Heat-shock gene expression and cell cycle changes during mammalian embryonic development.
    Dev Genet. 1993;14(2):127-36 PMID: 8482017
  58. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
    Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
  59. Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6911-5 PMID: 1871106
  60. Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Feb;11(2):1062-8 PMID: 1899282
  61. Heat shock (45 degrees C) results in an increase of nuclear matrix protein mass in HeLa cells.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Aug;50(2):253-68 PMID: 3488284
  62. The dynamics of a pre-mRNA splicing factor in living cells.
    Nature. 1997 May 29;387(6632):523-7 PMID: 9168118
  63. Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure.
    J Cell Biol. 1996 Dec;135(6 Pt 1):1427-40 PMID: 8978813
  64. Spheres, coiled bodies and nuclear bodies.
    Curr Opin Cell Biol. 1995 Jun;7(3):325-8 PMID: 7662361
  65. Heat shock transcription factors: structure and regulation.
    Annu Rev Cell Dev Biol. 1995;11:441-69 PMID: 8689565
  66. HSF1 granules: a novel stress-induced nuclear compartment of human cells.
    J Cell Sci. 1997 Dec;110 ( Pt 23):2925-34 PMID: 9359875
  67. Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity.
    EMBO J. 1997 Mar 17;16(6):1401-12 PMID: 9135155
  68. Molecular cloning and expression of a human heat shock factor, HSF1.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6906-10 PMID: 1871105
  69. Heat shock induces transient p53-dependent cell cycle arrest at G1/S.
    Oncogene. 1997 Jul 31;15(5):561-8 PMID: 9247309
  70. Heat-shock proteins Hsp104 and Hsp70 reactivate mRNA splicing after heat inactivation.
    Curr Biol. 1995 Mar 1;5(3):306-17 PMID: 7780741
  71. Cells in stress: transcriptional activation of heat shock genes.
    Science. 1993 Mar 5;259(5100):1409-10 PMID: 8451637
  72. Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries.
    Chromosome Res. 1993 Jul;1(2):93-106 PMID: 8143096
  73. Role of chromosome territories in the functional compartmentalization of the cell nucleus.
    Cold Spring Harb Symp Quant Biol. 1993;58:777-92 PMID: 7525149
  74. Splicing thermotolerance maintains Pre-mRNA transcripts in the splicing pathway during severe heat shock.
    Exp Cell Res. 1992 Oct;202(2):233-42 PMID: 1397078
  75. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2737-41 PMID: 6789322
  76. Structure and function in the nucleus.
    Science. 1998 Apr 24;280(5363):547-53 PMID: 9554838
  77. Of coiled bodies, gems, and salmon.
    J Cell Biochem. 1998 Aug 1;70(2):181-92 PMID: 9671224
  78. The molecular chaperones in cell cycle control.
    Ann N Y Acad Sci. 1998 Jun 30;851:61-6 PMID: 9668606
  79. Heat shock alters poly(A) tail length of Dictyostelium discoideum hsp32 RNA.
    DNA Cell Biol. 1998 Jul;17(7):635-41 PMID: 9703022
  80. RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis.
    Cell. 1986 Apr 25;45(2):185-93 PMID: 2421918
  81. Heat-induced modifications in the association of specific proteins with the nuclear matrix.
    Radiat Res. 1996 Jun;145(6):746-53 PMID: 8643835
  82. Displacement of sequence-specific transcription factors from mitotic chromatin.
    Cell. 1995 Oct 6;83(1):29-38 PMID: 7553870
  83. Associations between distinct pre-mRNA splicing components and the cell nucleus.
    EMBO J. 1991 Nov;10(11):3467-81 PMID: 1833187
  84. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
    Cell. 1982 Sep;30(2):517-28 PMID: 6814763
Article Info
Journal
Gene expression
Abbr.
Gene Expr
ISSN
1052-2166
Published
1999-00-00
Pages
261-70
Language
English
Region
United States
NLM ID
9200651
PMCID
PMC6174671
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038109 · United States
NIGMS NIH HHS · R37 GM038109 · United States
NIGMS NIH HHS · GM38109 · United States
External Links
PubMed source PMC Full Text View journal details
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