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PMID: 3437895 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induced heat shock mRNAs escape the nucleocytoplasmic transport block in adenovirus-infected HeLa cells.

Molecular and cellular biology ·Vol. 7 ·No. 12 ·1987-12-00 ·Pages 4505-12

Moore M, Schaack J, Baim SB, Morimoto RI, Shenk T

Abstract

Under conditions in which cytoplasmic accumulation of HeLa cell mRNAs has been blocked by adenovirus infection, hsp70 family mRNAs are transported from the nucleus to the cytoplasm at near normal efficiency subsequent to heat shock. Heat shock does not reverse the general virus-induced block to host cell mRNA transport. The heat shock mRNAs are translated within the cytoplasm of the infected cell but at substantially reduced efficiency compared with that of uninfected cells. Thus, the hsp70 family of mRNAs can escape the transport block but not the translational block instituted late after adenovirus infection. The beta-tubulin gene family is induced by the viral E1A gene after infection, and its mRNAs also accumulate in the cytoplasmic compartment. Given these two examples, it seems likely that the process of transcriptional induction allows the resulting mRNA to escape the viral block of transport.

MeSH Terms
Adenoviridae/genetics,physiology Biological Transport Cell Nucleus/metabolism Cytoplasm/metabolism HeLa Cells Heat-Shock Proteins/genetics Hot Temperature Humans Kinetics Nucleic Acid Hybridization Protein Biosynthesis RNA Splicing RNA, Messenger/genetics,metabolism RNA, Viral Transcription, Genetic Tubulin/genetics
Chemicals
Heat-Shock Proteins RNA, Messenger RNA, Viral Tubulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moore M
Department of Molecular Biology, Princeton University, New Jersey 08544.
Schaack J
Baim S B
Morimoto R I
Shenk T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.12.4505-4512.1987
Published
1987-12-00
Pages
4505-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368135
Subset
IM
Grants
NCI NIH HHS · CA 41086 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.12.4505-4512.1987 PMC Full Text View journal details
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