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PMID: 2943984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence-independent autoregulation of the adenovirus type 5 E1A transcription unit.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 3214-21

Hearing P, Shenk T

Abstract

The adenovirus E1A gene is known to be autoregulated at the level of transcription. Autoregulation was found to be mediated by products of the E1A 13S mRNA, which induced a fivefold increase in E1A transcription rate. Deletion analysis suggested that the autoregulation did not require any specific sequence in the E1A transcriptional control region. This conclusion was reinforced by the demonstration that a cellular alpha-globin gene substituted for the E1A gene on the adenovirus chromosome was also positively regulated by E1A gene products.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Cell Line Chromosome Deletion Genes Genes, Viral Globins/genetics HeLa Cells/metabolism Homeostasis Humans Kidney Mutation Oncogene Proteins, Viral/genetics Plasmids RNA, Messenger/genetics Transcription, Genetic Uridine/metabolism
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Messenger Globins Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hearing P
Shenk T
References (45)
45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.3214-3221.1985
Published
1985-11-00
Pages
3214-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369137
Subset
IM
Grants
NIAID NIH HHS · AI20412 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.11.3214-3221.1985 PMC Full Text View journal details
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