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PMID: 11894912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The E1b promoter of Ad12 in mouse L tk- cells is activated by adenovirus region E1a.

The EMBO journal ·Vol. 2 ·No. 1 ·1983-00-00 ·Pages 73-6

Bos JL, ten Wolde-Kraamwinkel HC

Abstract

We have investigated the effect of the E1a region of adenovirus on the expression of the E1b transcriptional unit in mouse L tk- cells. To that end we have fused the promoter region of the E1b gene of Ad12 to the coding sequence of the thymidine kinase gene of herpes simplex virus type 1. We found that expression of this fusion gene is completely dependent on the presence of the E1a region. Sequences involved in this regulation were mapped between positions -135 and +11 from the E1b cap site. In addition, we found that the largest E1a protein is essential in this regulation process.

MeSH Terms

Adenovirus E1A Proteins/metabolism Adenovirus E1B Proteins/genetics Adenoviruses, Human/genetics Animals Gene Expression Regulation, Viral Genes, Viral Humans L Cells Mice Promoter Regions, Genetic Transcriptional Activation

Chemicals

Adenovirus E1A Proteins Adenovirus E1B Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bos J L

Department of Medical Biochemistry, University of Leiden, The Netherlands.

ten Wolde-Kraamwinkel H C

References (14)

14 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

73-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555089

Subset

External Links

PubMed source PMC Full Text View journal details

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The E1b promoter of Ad12 in mouse L tk- cells is activated by adenovirus region E1a.

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