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PMID: 8264624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative 5' splice site selection induced by heat shock.

Molecular and cellular biology ·Vol. 14 ·No. 1 ·1994-01-00 ·Pages 567-75

Takechi H, Hosokawa N, Hirayoshi K, Nagata K

Abstract

The mouse HSP47 gene consists of six exons separated by five introns. Three HSP47 cDNAs differing only in their 5' noncoding regions have been reported. One of these alternatively spliced mRNAs was detected only after heat shock, which caused an alternative 5' splice donor site selection. Other stress inducers, including an amino acid analog and sodium arsenite, had no effect on the alternative splicing. The alternatively spliced mRNA, which was 169 nucleotides longer in the 5' noncoding region compared to mRNA transcribed in non-heat shock conditions, was efficiently translated under heat shock conditions. This novel finding that alternative splicing is caused by artificial treatment like heat shock will provide a useful in vivo model for understanding the exon-intron recognition mechanism as well as heat shock-induced alterations in gene expression.

MeSH Terms
3T3 Cells/metabolism Alternative Splicing/genetics Animals Base Sequence Binding Sites/genetics Biological Transport, Active Cytoplasm/metabolism DNA, Complementary/genetics Exons HSP47 Heat-Shock Proteins Heat-Shock Proteins/genetics Hot Temperature Introns Mice Models, Genetic Molecular Sequence Data Protein Biosynthesis RNA, Messenger/metabolism Transfection Tumor Cells, Cultured/metabolism
Chemicals
DNA, Complementary HSP47 Heat-Shock Proteins Heat-Shock Proteins RNA, Messenger Serpinh1 protein, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takechi H
Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan.
Hosokawa N
Hirayoshi K
Nagata K
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40 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.1.567-575.1994
Published
1994-01-00
Pages
567-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358406
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.1.567-575.1994 PMC Full Text View journal details
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