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PMID: 101519 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell envelope alterations in antibiotic-sensitive and-resistant strains of Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 136 ·No. 1 ·1978-10-00 ·Pages 391-401

Guymon LF, Walstad DL, Sparling PF

Abstract

The cell envelopes of antibiotic-resistant and -sensitive isogenic strains of Neisseria gonorrhoeae were analyzed to determine whether acquisition of genetic loci for altered antibiotic sensitivity was accompanied by alterations in cell envelope composition. No differences in the composition of phospholipids and lipopolysaccharides were noted. Acquisition of mtr-2, which results in low-level, nonspecific increased resistance to multiple antibiotics, dyes, and detergents, was accompanied by a sevenfold increase in the amount of a minor, 52,000-molecular-weight outer membrane protein and a 32% increase in the extent of peptidoglycan cross-linking. Subsequent addition of the nonspecific hypersensitivity loci env-1 or env-2 to a strain carrying mtr-2 resulted in reversal of the phenotypic resistance determined by mtr-2 and marked reduction in both the amount of the 52,000-molecular-weight outer membrane protein and the extent of peptidoglycan cross-linking. Introduction of penB2, which results in a fourfold increase in resistance to penicillin and tetracycline, was accompanied by the disappearance of the principal outer membrane protein of the wild-type strain (molecular weight, 36,900) and the appearance of a new species of the principal outer membrane protein (molecular weight, 39,400) in the transformant.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/metabolism Cell Membrane/metabolism Drug Resistance, Microbial Genes Lipopolysaccharides/analysis Membrane Proteins/metabolism Neisseria gonorrhoeae/drug effects,ultrastructure Peptidoglycan/metabolism Phospholipids/analysis Polysaccharides, Bacterial/analysis
Chemicals
Anti-Bacterial Agents Bacterial Proteins Lipopolysaccharides Membrane Proteins Peptidoglycan Phospholipids Polysaccharides, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guymon L F
Walstad D L
Sparling P F
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54 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.1.391-401.1978
Published
1978-10-00
Pages
391-401
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218671
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.1.391-401.1978 PMC Full Text View journal details
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