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PMID: 821921 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 127 ·No. 2 ·1976-08-00 ·Pages 874-80

Senff LM, Wegener WS, Brooks GF, Finnerty WR, Makula RA

Abstract

Exponential-phase cells of Neisseria gonorrhaeae 2686 were examined for phospholipid composition and for membrane-associated phospholipase A activity. When cells were harvested by centrifugation, washed, and lyophilized before extraction, approximately 74% of the total phospholipid was phosphatidylethanolamine, 18% was phosphatidylglycerol, 2% was cardiolipin, and 10% was lysophosphatidylethanolamine. However, when cells still suspended in growth medium were extracted, the amount of lysophosphatidylethanolamine decreased to approximately 1% of the phospholipid composition. This suggests that a gonococcal phospholipase A may be activated by conditions encountered during centrifugation and/or lyophilization of cells preceding extraction. Phospholipase A activity associated with cell membranes was assayed by measuring the conversion of tritiated phosphatidylethanolamine to lysophosphatidylethanolamine. Optimal activity was demonstrated in 10% methanol at pH 8.0 to 8.5, in the presence of calcium ions. The activity was both detergent sensitive and thermolabile. Comparisons of gonococcal colony types 1 and 4 showed no significant differences between the two types with respect to either phospholipid content or phospholipase A activity.

MeSH Terms
Calcium/pharmacology Cardiolipins/analysis Cell Membrane/enzymology Cell-Free System Neisseria gonorrhoeae/analysis,enzymology Phosphatidylethanolamines/analysis Phosphatidylglycerols/analysis Phospholipases/metabolism Phospholipids/analysis,metabolism
Chemicals
Cardiolipins Phosphatidylethanolamines Phosphatidylglycerols Phospholipids Phospholipases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Senff L M
Wegener W S
Brooks G F
Finnerty W R
Makula R A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.2.874-880.1976
Published
1976-08-00
Pages
874-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC232996
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.2.874-880.1976 PMC Full Text View journal details
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