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PMID: 10022842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.

The EMBO journal ·Vol. 18 ·No. 4 ·1999-02-15 ·Pages 1003-13

Vader A, Nielsen H, Johansen S

Abstract

The Didymium iridis DiSSU1 intron is located in the nuclear SSU rDNA and has an unusual twin-ribozyme organization. One of the ribozymes (DiGIR2) catalyses intron excision and exon ligation. The other ribozyme (DiGIR1), which along with the endonuclease-encoding I-DirI open reading frame (ORF) is inserted in DiGIR2, carries out hydrolysis at internal processing sites (IPS1 and IPS2) located at its 3' end. Examination of the in vivo expression of DiSSU1 shows that after excision, DiSSU1 is matured further into the I-DirI mRNA by internal DiGIR1-catalysed cleavage upstream of the ORF 5' end, as well as truncation and polyadenylation downstream of the ORF 3' end. A spliceosomal intron, the first to be reported within a group I intron and the rDNA, is removed before the I-DirI mRNA associates with the polysomes. Taken together, our results imply that DiSSU1 uses a unique combination of intron-supplied ribozyme activity and adaptation to the general RNA polymerase II pathway of mRNA expression to allow a protein to be produced from the RNA polymerase I-transcribed rDNA.

MeSH Terms
Amoeba/genetics Animals Base Sequence Cloning, Molecular DNA, Ribosomal/genetics Gene Expression Regulation/genetics Introns/genetics Molecular Sequence Data Open Reading Frames/genetics Polyribosomes/genetics RNA Polymerase II/genetics RNA Precursors/genetics RNA Processing, Post-Transcriptional/genetics RNA Splicing/genetics RNA, Catalytic/genetics RNA, Messenger/genetics Sequence Analysis, DNA Sequence Analysis, RNA Spliceosomes/genetics
Chemicals
DNA, Ribosomal RNA Precursors RNA, Catalytic RNA, Messenger RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vader A
Department of Molecular Cell Biology, Institute of Medical Biology, University of Tromso, N-9037 Tromso, Norway. annav@fagmed.uit.no
Nielsen H
Johansen S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.4.1003
Email
annav@fagmed.uit.no
Published
1999-02-15
Pages
1003-13
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171192
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.4.1003 PMC Full Text View journal details
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