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PMID: 7556099 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two group I ribozymes with different functions in a nuclear rDNA intron.

The EMBO journal ·Vol. 14 ·No. 18 ·1995-09-15 ·Pages 4558-68

Decatur WA, Einvik C, Johansen S, Vogt VM

Abstract

DiSSU1, a mobile intron in the nuclear rRNA gene of Didymium iridis, was previously reported to contain two independent catalytic RNA elements. We have found that both catalytic elements, renamed GIR1 and GIR2, are group I ribozymes, but with differing functionality. GIR2 carries out the several reactions associated with self-splicing. GIR1 carries out a hydrolysis reaction at an internal processing site (IPS-1). These conclusions are based on the catalytic properties of RNAs transcribed in vitro. Mutation of the P7 pairing segment of GIR2 abrogated self-splicing, while mutation of P7 in GIR1 abrogated hydrolysis at the IPS-1. Much of the P2 stem and all of the associated loop could be deleted without effect on self-splicing. These results are accounted for by a secondary structure model, in which a long P2 pairing segment brings the 5' splice site to the GIR2 catalytic core. GIR1 is the smallest natural group I ribozyme yet reported and is the first example of a group I ribozyme whose presumptive biological function is hydrolysis. We hypothesize that GIR1-mediated cleavage of the excised intron RNA functions in the generation and expression of the mRNA for the intron-encoded endonuclease I-DirI.

MeSH Terms
Base Sequence Catalysis Cell Compartmentation Cell Nucleus/genetics DNA Mutational Analysis DNA, Ribosomal/genetics Introns Models, Genetic Molecular Sequence Data Myxomycetes/genetics Nucleic Acid Conformation Polymerase Chain Reaction RNA Splicing RNA, Catalytic/classification,genetics,metabolism RNA, Ribosomal/genetics,metabolism Sequence Deletion
Chemicals
DNA, Ribosomal RNA, Catalytic RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Decatur W A
Section of Biochemistry, Molecular and Cellular Biology, Cornell University, Ithaca, NY 14853, USA.
Einvik C
Johansen S
Vogt V M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-09-15
Pages
4558-68
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394548
Subset
IM
Grants
NIGMS NIH HHS · GM51860 · United States
External Links
PubMed source PMC Full Text View journal details
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