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PMID: 7984404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleolar introns from Physarum flavicomum contain insertion elements that may explain how mobile group I introns gained their open reading frames.

Nucleic acids research ·Vol. 22 ·No. 22 ·1994-11-11 ·Pages 4553-9

Vader A, Naess J, Haugli K, Haugli F, Johansen S

Abstract

Comparison of two group I intron sequences in the nucleolar genome of the myxomycete Physarum flavicomum to their homologs in the closely related Physarum polycephalum revealed insertion-like elements. One of the insertion-like elements consists of two repetitive sequence motifs of 11 and 101 bp in five and three copies, respectively. The smaller motif, which flanks the larger, resembles a target duplication and indicates a relationship to transposons or retroelements. The insertion-like elements are found in the peripheral loops of the RNA structure; the positions occupied by the ORFs of mobile nucleolar group I introns. The P. flavicomum introns are 1184 and 637 bp in size, located in the large subunit ribosomal RNA gene, and can be folded into group I intron structures at the RNA level. However, the intron 2s from both P. flavicomum and P. polycephalum contain an unusual core region that lacks the P8 segment. None of the introns are able to self-splice in vitro. Southern analysis of different isolates indicates that the introns are not optional in myxomycetes.

MeSH Terms
Animals Base Sequence Cell Nucleolus/genetics Cloning, Molecular DNA Transposable Elements/genetics DNA, Fungal/analysis DNA, Ribosomal/analysis Introns/genetics Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames/genetics Physarum/genetics RNA, Fungal/analysis,chemistry RNA, Ribosomal/analysis,chemistry Repetitive Sequences, Nucleic Acid/genetics Sequence Alignment Sequence Analysis, DNA
Chemicals
DNA Transposable Elements DNA, Fungal DNA, Ribosomal RNA, Fungal RNA, Ribosomal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vader A
Department of Cell Biology, University of Tromsø, Norway.
Naess J
Haugli K
Haugli F
Johansen S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.22.4553
Published
1994-11-11
Pages
4553-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308500
Subset
IM
Databases
GENBANK
X78959
External Links
PubMed source DOI: 10.1093/nar/22.22.4553 PMC Full Text View journal details
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