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PMID: 8127708 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba.

Nucleic acids research ·Vol. 22 ·No. 4 ·1994-02-25 ·Pages 592-6

Gast RJ, Fuerst PA, Byers TJ

Abstract

The discovery of group I introns in small subunit nuclear rDNA (nsrDNA) is becoming more common as the effort to generate phylogenies based upon nsrDNA sequences grows. In this paper we describe the discovery of the first two group I introns in the nsrDNA from the genus Acanthamoeba. The introns are in different locations in the genes, and have no significant primary sequence similarity to each other. They are identified as group I introns by the conserved P, Q, R and S sequences (1), and the ability to fit the sequences to a consensus secondary structure model for the group I introns (1, 2). Both introns are absent from the mature srRNA. A BLAST search (3) of nucleic acid sequences present in GenBank and EMBL revealed that the A. griffini intron was most similar to the nsrDNA group I intron of the green alga Dunaliella parva. A similar search found that the A. lenticulata intron was not similar to any of the other reported group I introns.

MeSH Terms
Acanthamoeba/genetics Animals Base Sequence Genes, Protozoan/genetics Introns/genetics Molecular Sequence Data Nucleic Acid Conformation Polymerase Chain Reaction RNA, Protozoan/genetics RNA, Ribosomal/genetics
Chemicals
RNA, Protozoan RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gast R J
Department of Molecular Genetics, Ohio State University, Columbus 43210-1292.
Fuerst P A
Byers T J
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.4.592
Published
1994-02-25
Pages
592-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307848
Subset
IM
Databases
GENBANK
U02539, U02540, U07412
External Links
PubMed source DOI: 10.1093/nar/22.4.592 PMC Full Text View journal details
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