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PMID: 9742098 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.

Molecular and cellular biology ·Vol. 18 ·No. 10 ·1998-10-00 ·Pages 5809-17

Lin J, Vogt VM

Abstract

PpLSU3, a mobile group I intron in the rRNA genes of Physarum polycephalum, also can home into yeast chromosomal ribosomal DNA (rDNA) (D. E. Muscarella and V. M. Vogt, Mol. Cell. Biol. 13:1023-1033, 1993). By integrating PpLSU3 into the rDNA copies of a yeast strain temperature sensitive for RNA polymerase I, we have shown that the I-PpoI homing endonuclease encoded by PpLSU3 is expressed from an RNA polymerase I transcript. We have also developed a method to integrate mutant forms of PpLSU3 as well as the Tetrahymena intron TtLSU1 into rDNA, by expressing I-PpoI in trans. Analysis of I-PpoI expression levels in these mutants, along with subcellular fractionation of intron RNA, strongly suggests that the full-length excised intron RNA, but not RNAs that are further cleaved, serves as or gives rise to the mRNA.

MeSH Terms
Animals Binding Sites DNA Transposable Elements DNA, Ribosomal Endodeoxyribonucleases/genetics,metabolism Gene Expression Introns Mutagenesis Nucleic Acid Conformation Physarum/metabolism RNA/metabolism RNA Polymerase I/genetics Saccharomyces cerevisiae/genetics,metabolism Tetrahymena/genetics
Chemicals
DNA Transposable Elements DNA, Ribosomal RNA RNA Polymerase I Endodeoxyribonucleases I-Ppo endonuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin J
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
Vogt V M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.10.5809
Published
1998-10-00
Pages
5809-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109167
Subset
IM
Grants
NIGMS NIH HHS · GM-51860 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.10.5809 PMC Full Text View journal details
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