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PMID: 1461736 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The histone mRNA 3' end is required for localization of histone mRNA to polyribosomes.

Nucleic acids research ·Vol. 20 ·No. 22 ·1992-11-25 ·Pages 6057-66

Sun J, Pilch DR, Marzluff WF

Abstract

The final step in mRNA biosynthesis is transport of the mRNA from the nucleus to the cytoplasm. Histone genes from which the 3' stem-loop has been deleted are transcribed to give RNAs with heterogeneous 3' ends. These RNAs are localized in the nucleus and are stable. Addition of the histone 3' processing signal either on short (< 250 nts) or long (> 1000 nts) transcripts restores 3' processing and transport of the mRNA to the cytoplasm. In addition chimeric histone-U1 snRNA genes which produced RNAs with either histone or U1 3' ends were analyzed. Transcripts which ended with U1 snRNA 3' ends were not efficiently localized to polyribosomes. However, transcripts containing the same sequences including the snRNA 3' end followed by the histone 3' end were present in the cytoplasm on polyribosomes. Taken together these results suggest that the histone 3' end is required for export of histone mRNA to the cytoplasm and association of the mRNA with polyribosomes.

MeSH Terms
Animals Biological Transport CHO Cells Cell Nucleus/metabolism Cricetinae Cytoplasm/metabolism Histones/genetics L Cells Mice Poly A/metabolism Polyribosomes/metabolism RNA, Messenger/metabolism Restriction Mapping Transcription, Genetic
Chemicals
Histones RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sun J
Institute of Molecular Biophysics, Florida State University, Tallahassee 32306.
Pilch D R
Marzluff W F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.22.6057
Published
1992-11-25
Pages
6057-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334473
Subset
IM
Grants
NIGMS NIH HHS · GM29832 · United States
External Links
PubMed source DOI: 10.1093/nar/20.22.6057 PMC Full Text View journal details
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