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PMID: 3386629 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 1887-95

Levine BJ, Liu TJ, Marzluff WF, Skoultchi AI

Abstract

Histone proteins are encoded by a multigene family. The H3.2(614) and H2a(614) genes are present as single copies which are expressed at high levels, accounting for 30 to 40% of the H3 and H2a mRNAs, respectively, in different types of mouse cells. The other genes which have been isolated each contribute only a very small amount to the total type-specific mRNA pool. We demonstrate here that the differences in the level of expression of these genes are partly due to differences in their transcription rates. To investigate the sequences responsible for these differences in expression among the members of each family, we carried out DNA-mediated gene transfer experiments with both intact and chimeric histone genes. The 5' region of a highly expressed gene [H3.2(614) or H2a(614)] was attached to the 3' region of a histone gene which was expressed at low levels (H3-221 or H2a-291) and vice versa. The results show that sequences in both the 5' and 3' regions of the H3.2(614) and H2a(614) genes contribute to their high level of mRNA production by two independent mechanisms. The effect of the 3' sequences on mRNA accumulation has been narrowed to a 65-base-pair region including the 3'-terminal palindrome and downstream signal implicated in mRNA processing.

MeSH Terms
Animals Cell Line Cricetinae Cricetulus Female Histones/biosynthesis,genetics Mice/genetics Multigene Family Ovary RNA, Messenger/biosynthesis Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Histones RNA, Messenger Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Levine B J
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Liu T J
Marzluff W F
Skoultchi A I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.1887-1895.1988
Published
1988-05-00
Pages
1887-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363366
Subset
IM
Grants
PHS HHS · 29832 · United States
NCI NIH HHS · CA13330 · United States
NCI NIH HHS · CA16368 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.5.1887-1895.1988 PMC Full Text View journal details
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