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PMID: 8524251 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Increased expression of LD1 genes transcribed by RNA polymerase I in Leishmania donovani as a result of duplication into the rRNA gene locus.

Molecular and cellular biology ·Vol. 15 ·No. 12 ·1995-12-00 ·Pages 6845-53

Lodes MJ, Merlin G, deVos T, Ghosh A, Madhubala R, Myler PJ, Stuart K

Abstract

Eukaryotic protein-coding genes are generally transcribed by RNA polymerase II (Pol II), which has a lower transcription rate than that of Pol I. We report here the duplication of two LD1 genes into the rRNA locus and their resultant transcription by Pol I. The multigenic LD1 locus is present in a 2.2-Mb chromosome in all stocks of Leishmania spp. and is also present in multicopy 200- to 450-kb linear chromosomes or multicopy circular DNAs in over 15% of stocks examined. Genomic rearrangement in Leishmania donovani LSB-51.1 resulted in duplication of a 3.9-kb segment of LD1 containing two genes (orfF and orfG) and of a 1.3-kb segment from approximately 10 kb downstream into the rRNA gene repeat region of the 1.2-Mb chromosome. Short sequences (12 or 13 bp) common to the 2.2-Mb LD1 and 1.2-Mb rRNA loci suggest that this gene conversion occurred by homologous recombination. Transcription of the duplicated genes is alpha-amanitin resistant, indicating transcription by Pol I, in contrast to the alpha-amanitin-sensitive (Pol II) transcription of the genes in the 2.2-Mb LD1 locus. This results in higher transcript abundance than expected from the gene copy number in LSB-51.1 and in elevated expression of at least the orfF gene product.

MeSH Terms
Amanitins/pharmacology Animals Base Sequence DNA Primers Gene Expression Gene Library Genes, Protozoan Leishmania donovani/genetics,metabolism Molecular Sequence Data Multigene Family Open Reading Frames Polymerase Chain Reaction RNA, Protozoan/genetics RNA, Ribosomal/genetics Recombination, Genetic Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic/drug effects
Chemicals
Amanitins DNA Primers RNA, Protozoan RNA, Ribosomal
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lodes M J
Seattle Biomedical Research Institute, Washington 98109-1651, USA.
Merlin G
deVos T
Ghosh A
Madhubala R
Myler P J
Stuart K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.12.6845
Published
1995-12-00
Pages
6845-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230939
Subset
IM
Grants
NIAID NIH HHS · AI08815 · United States
NIAID NIH HHS · AI17375 · United States
Databases
GENBANK
L38570, L38571, L38572
External Links
PubMed source DOI: 10.1128/MCB.15.12.6845 PMC Full Text View journal details
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